An improved process for producing outer membrane vesicles
An outer membrane vesicle, physiological technology, applied in the field of medical microbiology and vaccinology, can solve the problems of cell death bacteria, lysis and so on
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[0016] We have unexpectedly found that the spontaneous release of outer membrane vesicles (OMVs) can be triggered directly and reliably using common control parameters of bioreactor culture.
[0017] In a first aspect, the present invention relates to a method of producing spontaneously released bacterial outer membrane vesicles. Preferably, the method comprises the step of: a) culturing the Gram-negative bacterial population comprising stimulating OMV release by applying a DOT higher than the physiological dissolved oxygen tension (DOT) of 30%. The method also preferably comprises the step of: b) recovering the OMVs released in a), wherein preferably the recovering comprises at least removing bacteria from the OMVs.
[0018] As used herein, the term OMV ("outer membrane vesicle") denotes a released outer membrane sphere with a periplasmic content comprising bioactive molecules (toxins, protein, DNA). OMV is also sometimes referred to as "bleb". These vesicles are often inv...
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