Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

An improved process for producing outer membrane vesicles

An outer membrane vesicle, physiological technology, applied in the field of medical microbiology and vaccinology, can solve the problems of cell death bacteria, lysis and so on

Pending Publication Date: 2020-07-17
INTRAVACC有限责任公司
View PDF8 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the addition of hydrogen peroxide is not feasible for a scalable OMV production method because the addition of hydrogen peroxide to bacterial cultures results in significant cell death and bacterial lysis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • An improved process for producing outer membrane vesicles
  • An improved process for producing outer membrane vesicles
  • An improved process for producing outer membrane vesicles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] We have unexpectedly found that the spontaneous release of outer membrane vesicles (OMVs) can be triggered directly and reliably using common control parameters of bioreactor culture.

[0017] In a first aspect, the present invention relates to a method of producing spontaneously released bacterial outer membrane vesicles. Preferably, the method comprises the step of: a) culturing the Gram-negative bacterial population comprising stimulating OMV release by applying a DOT higher than the physiological dissolved oxygen tension (DOT) of 30%. The method also preferably comprises the step of: b) recovering the OMVs released in a), wherein preferably the recovering comprises at least removing bacteria from the OMVs.

[0018] As used herein, the term OMV ("outer membrane vesicle") denotes a released outer membrane sphere with a periplasmic content comprising bioactive molecules (toxins, protein, DNA). OMV is also sometimes referred to as "bleb". These vesicles are often inv...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
absorbanceaaaaaaaaaa
refractive indexaaaaaaaaaa
Login to View More

Abstract

The present invention relates to the fields of medical microbiology and vaccines. In particular the invention relates to a process wherein the spontaneous release of bacterial outer membrane vesicles(OMV) of Gram-negative bacteria is stimulated by application of a dissolved oxygen tension (DOT) that is higher than a physiological DOT. The thus produced OMVs are for use in vaccines. The inventionfurther relates to OMV obtainable by said process, and to a pharmaceutical composition comprising such OMV. The present invention further relates to the use of OMV of the present invention as a medicament in particular for use in a method for eliciting an immune response.

Description

technical field [0001] The invention relates to the fields of medical microbiology and vaccinology. In particular, the present invention relates to a method for producing Gram-negative bacterial spontaneous outer membrane vesicles (OMVs) for use in vaccines, OMVs obtainable by said method, and pharmaceutical compositions comprising such OMVs. The invention also relates to the use of an OMV according to the invention as a medicament, in particular in a method of eliciting an immune response. Background technique [0002] Outer membrane vesicles (OMVs) are naturally produced by Gram-negative bacteria and are involved in pathogenesis, intercellular communication, and stress response (Kulp and Kuehn 2010). Recent studies have shown membrane vesicle formation in Gram-positive bacteria and archaea (Ellen et al. 2009; Rivera et al. 2010). OMVs are spherical nanoparticles, vesicles composed of a phospholipid bilayer containing proteins and lipopolysaccharide (LPS), and the vesicle...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04C12P21/02A61K39/095A61K39/02A61K39/108
CPCA61K39/0208A61K39/0258A61K39/095A61K39/099C12P21/02C12N1/20C12P1/04Y02A50/30C12M41/34A61P31/00C12N2509/10A61K2039/55555
Inventor M·J·H·格里岑L·A·范德波尔M·施托克
Owner INTRAVACC有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com