Colony counting method based on machine vision

Abstract

The invention provides a bacterial colony counting method based on machine vision in the field of bacterial colony counting, which comprises the following steps: step S10, acquiring an original photoof a culture dish, and preprocessing the original photo to generate an optimized photo; S20, traversing and searching a first outline of a culture dish in the optimized photo, setting an area outsidethe first outline to be black, and generating a photograph of the culture dish; S30, traversing and searching a second outline of each bacterial colony in the photograph; and step S40, counting the second contours. The bacterial colony counting device has the advantage that the bacterial colony counting efficiency and precision are greatly improved.

Description

technical field [0001] The invention relates to the field of colony counting, in particular to a method for counting colonies based on machine vision. Background technique [0002] The determination of the total number of bacterial colonies is used to determine the degree of food contamination by bacteria and the hygienic quality. It reflects whether the food meets the hygienic requirements during the production process, so as to make an appropriate hygienic evaluation of the tested samples. The total number of bacterial colonies can be determined to a certain extent. It marks the quality of food hygiene. In order to count the colonies, the sample to be tested needs to be diluted first, and then poured on a petri dish for cultivation, and the colonies are counted after culturing for a certain period of time. [0003] Traditionally, for the counting of colonies, the method of manually dotting the lid of the petri dish with a marker pen is used, and then the statistics are pe...

AI Technical Summary

Problems solved by technology

[0003] Traditionally, for the counting of colonies, the method of manually dotting the lid of the petri dish with a marker pen is used, and then the statistics are performed after the dots are completed. The traditional method is easy to cause fatigue, and the dot process is easily disturbed by the external environment, making different people Or the counting results of different times are different, and the counting efficiency is low; although there are colony counters on the market, it is only a simple replacement of the marker pen with an electronic pen, which still needs to be done manually. fundamentally solve the problem

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