Hematoxylin staining solution oxidation degree monitoring method and a hematoxylin staining solution quality control method

Abstract

The invention relates to a hematoxylin staining solution oxidation degree monitoring method and a hematoxylin staining solution quality control method, and belongs to the technical field of hematoxylin staining solutions. The hematoxylin staining solution oxidation degree monitoring method comprises the following steps: (1) detecting an OD value of a diluent of the hematoxylin staining solution before oxidation by using an ultraviolet spectrophotometer, and recording the OD value as OD0; (2) detecting the OD value of the diluent of the oxidized hematoxylin staining solution by using an ultraviolet spectrophotometer, and recording the OD value as ODt; (3) before and after oxidation, observing that the OD value of the diluent of the hematoxylin staining solution changes regularly, and the change rule is as follows: the larger the oxidation degree is, the larger the numerical value of the OD value is; monitoring the oxidation degree of the hematoxylin staining solution according to the increase degree of the ODt relative to the OD0. The quality control method is rapid, accurate and easy to popularize and use. The method solves the problem that the quality of dyed tablets is always baddue to the fact that the quality of the existing hematoxylin staining solution cannot be controlled.

Description

technical field [0001] The invention relates to a monitoring method for the oxidation degree of a hematoxylin staining liquid and a quality control method for the hematoxylin staining liquid, belonging to the technical field of the hematoxylin staining liquid. Background technique [0002] Hematoxylin-Eosin staining (Hematoxylin-Eosin staining), referred to as HE staining, is the most commonly used staining method in various hospital laboratories, and it is also the most classic staining technique in various pathology departments. The dyeing principle of HE staining method is as follows. The main component of chromatin in the nucleus is deoxyribose nucleic acid (DNA for short). In the double helix structure of DNA, the phosphate groups on the two chains are outward, negatively charged, acidic, and very It is easy to combine with positively charged hematoxylin basic dye to be stained by ion bond or hydrogen bond. Hematoxylin is blue in alkaline solution, so the cell nucleus i...

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Problems solved by technology

However, these two oxidation methods are not easy to grasp the degree of oxidation. If the degree of oxidation is not enough, the coloring will be slow and the dyeing power will be weak. If it is over-oxidized, it will easily form particle precipitation and surface metal oxide film, which will affect the dyeing effect and service life. The hematoxylin used The degree of oxidation of the staining solution will affect the quality of the stained film. If the oxidation degree of the hematoxylin staining solution cannot be confirmed, the quality of the stained film will be good or bad.

[0004] In order to improve the quality of the dyed film, it is necessary to obtain a quality-controllable hematoxylin-containing staining solution. At present, the strategies adopted mainly include: one strategy is to frequently adjust the dyeing time to remove the metal oxide film, and the other strategy is to It is necessary to prepare a new HE staining solution; both methods are cumbersome and affect work efficiency

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