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Intestinal flora for diagnosing sarcopenia and application of intestinal flora

A technology of intestinal flora and intestinal flora, which is applied in the field of intestinal flora in the diagnosis of sarcopenia, and can solve the problems of no uniform standard

Active Publication Date: 2020-07-14
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the diagnosis of sarcopenia, many studies have pointed out that it can be diagnosed by measuring grip strength, step count, etc., but there is no uniform standard (Yu S, Umapathysivam K, Visvanathan R. Sarcopenia in older people[J]. Int J Evid BasedHealthc,2014,12(4):227-243)

Method used

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  • Intestinal flora for diagnosing sarcopenia and application of intestinal flora
  • Intestinal flora for diagnosing sarcopenia and application of intestinal flora
  • Intestinal flora for diagnosing sarcopenia and application of intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Screening of intestinal flora associated with sarcopenia

[0036] 1. Research objects and sample collection

[0037] Stool samples were collected from 11 sarcopenic and 60 healthy individuals. The patient statistics are shown in Table 1.

[0038] Table 1 Patient Information

[0039]

[0040] 2. 16S rRNA sequencing

[0041] 2.1 Extraction of DNA

[0042] Genomic DNA was extracted from the samples using the Genomic DNA Extraction Kit, and the operation steps were carried out according to the instructions.

[0043] 2.2 DNA sample purity and concentration determination

[0044] Genomic DNA was detected by 1% agarose gel electrophoresis.

[0045] 2.3 PCR amplification and product purification

[0046] According to the designated sequencing region, synthesize specific primers with barcode, or synthesize fusion primers with misplaced bases.

[0047] PCR was performed using TransGen AP221-02: TransStart Fastpfu DNA Polymerase; all samples were carried out a...

Embodiment 2

[0065] Example 2 Verifies the correlation between Eisenbergiella and sarcopenia

[0066] 1. Research objects and sample collection

[0067] According to the method of Example 1, 11 samples of sarcopenia and 72 samples of healthy people were collected. The statistics of the patients are shown in Table 3.

[0068] Table 3 Patient Information

[0069]

[0070] 2. 16S rRNA sequencing and data analysis are the same as in Example 1.

[0071] 3. Results

[0072] The detection results showed that the abundance level of Eisenbergiella was significantly increased (P=3.72E-05). The results of the ROC curve showed that the AUC value was 0.799, the specificity of the optimal critical value was 0.764, and the sensitivity was 0.818 ( image 3 ), suggesting that the application of Eisenbergiella in the diagnosis of sarcopenia has a high diagnostic efficiency.

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Abstract

The invention discloses an intestinal flora for diagnosing sarcopenia and application of the intestinal flora. The intestinal flora is Eisenbergiella. Through 16S ribosomal ribonucleic acid sequencing, the situation that Eisenbergiella is remarkably increased in sarcopenia is found for a first time, the situation that high specificity and sensitivity can be achieved when sarcopenia and healthy people are distinguished by using Eisenbergiella as a detection variable is further found, so that on the basis, Eisenbergiella can be applied to noninvasive diagnosis on sarcopenia.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to intestinal flora for diagnosing sarcopenia and application thereof. Background technique [0002] Sarcopenia, referred to as sarcopenia, was first proposed by Evans WJ and Rosenberg IR in 1991, and has now received more and more attention. In 2010, the European Sarcopenia Working Group proposed the definition: sarcopenia is a progressive loss of muscle mass and strength that may lead to physical disability, reduced quality of life and even death (Cruz-Jentoft AJ, Baeyens JP , Bauer JM, et al. Sarcopenia: European consensus on definition and diagnosis: report of the European working group on sarcopenia in older people [J]. Age Aging, 2010, 39(4): 412-423.). Regarding the diagnosis of sarcopenia, many studies have pointed out that it can be diagnosed by measuring grip strength, step count, etc., but there is no uniform standard (Yu S, Umapathysivam K, Visvanathan R. Sarcopenia in older ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/689A61K45/00A61P21/00
CPCA61K45/00A61P21/00C12Q1/6883C12Q1/689C12Q2600/158
Inventor 康琳
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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