Method for promoting release of bonded aromatic substances in fruit juice
A technology of aroma substances and bonding state, applied in the field of plant food flavor, to achieve uniform and stable effect, increase variety, and overcome the effects of metallic taste and peculiar smell
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Embodiment 1
[0038] A method for releasing bonded aroma substances, the specific steps are as follows:
[0039] (1) Filter freshly squeezed orange juice through four layers of gauze, and then centrifuge for 15 minutes (4°C, 9000r / min) to obtain relatively clear supernatant juice;
[0040] (2) Flow the supernatant juice through a column (Φ2.2cm×50cm) filled with Amberlite XAD-2 resin at a flow rate of 3mL / min, and then wash the column with deionized water until the water is clear, in order to remove water-soluble sugar and acid class of substances. Then wash the column with 300mL ether / pentane (1:1, V / V) solution to remove free volatile substances, and then use 300mL methanol to remove the bonded volatile substances (glycosides) adsorbed on the Amberlite XAD-2 resin column substance) elutes. The methanol fraction was collected, concentrated to dryness under reduced pressure on a rotary evaporator (35°C, 0.8MPa), dissolved with 30mL 0.06mol / L citric acid-disodium hydrogen phosphate (pH 5) ...
Embodiment 2
[0046] Using the single factor experimental design method, the main research is to deal with different ultrasonic power density, ultrasonic time, ultrasonic temperature and ultrasonic duty cycle to release the bonded aroma substances of orange juice. Explore the law of the change of bonded aroma substances under different ultrasonic conditions. All the other steps and parameters are the same as in Example 1.
[0047] a) The effect of ultrasonic power density on the release of bonded aroma substances ( figure 1 )
[0048] The fixed ultrasonic time was 10 min, the temperature was 20°C, the duty cycle was 50%, and the ultrasonic power densities were 180, 360, 540, 720, and 900 W / L, respectively.
[0049] b) The effect of ultrasonic time on the release of bonded aroma substances ( figure 2 )
[0050] The ultrasonic power is fixed at 30%, that is, the ultrasonic power density is 540W / L, the temperature is 20°C, the duty cycle is 50%, and the ultrasonic time is 5, 10, 15, 20, a...
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