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Rapid germination method of tilia tuan seeds

A seed and linden tree technology, which is applied in the field of forest tree genetics and breeding, can solve the problems of irregular seed germination, low emergence rate, long seed dormancy time, etc., and achieve the effect of promoting seed germination

Inactive Publication Date: 2020-07-10
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The germination method of the present invention can solve the breeding technical problems such as long dormancy time of linden seeds, low emergence rate, irregular seed germination, etc.

Method used

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  • Rapid germination method of tilia tuan seeds
  • Rapid germination method of tilia tuan seeds
  • Rapid germination method of tilia tuan seeds

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Experimental program
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Effect test

Embodiment 1

[0039] The air-dried seeds of Tilia japonica collected in the field germinate rapidly.

[0040] S1, removing the linden seed pericarp by rolling. Put the seeds into boiling water at 100°C for 10 seconds. Then turn to 25 ℃ sterile water to soak for 24 hours.

[0041] S2, preparing a sterile solution of 0.4 μm MABA receptor inhibitor (ARA), and soaking the absorbent paper in the petri dish.

[0042] S3, then transfer the linden tree seeds with imbibition to a petri dish. The ambient temperature was chosen to be 20°C, and the culture was carried out in the dark for 4 days.

[0043] S4, then transferred to 4 ° C environment, dark culture, 3 days.

[0044] S5, the seeds are transferred into plug trays, and the ratio of the plug tray culture medium is perlite: vermiculite: peat=1:1:1.

[0045]Utilizing the rapid germination method of linden seeds of the present invention (ABA receptor de-inhibition method), the germination rate of linden seeds is 93.3%, and the seeds germinate ...

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Abstract

The present invention relates to a rapid germination method of tilia tuan seeds and belongs to the technical field of forest tree genetic breeding. The germination method comprises the following steps: 1) treating tilia tuan seeds at 90-100 DEG C for 2-10 s and soaking the tilia tuan seeds in water at 24-26 DEG C for 24-48 h; 2) conducting a first dark culture of the swollen seeds in an abscisic acid acceptor inhibitor; 3) conducting a second dark culture of the tilia tuan seeds after the first dark culture; and 4) transferring the seeds after the second dark culture to a substrate for cultivation. The germination method can complete germination of the tilia tuan seeds at low cost, quickly and efficiently, and provides a strong technical support for preservation of tilia tuan germplasm resources and selection and breeding of excellent new varieties.

Description

technical field [0001] The invention relates to the technical field of tree genetics and breeding, in particular to a method for rapid germination of linden seeds. Background technique [0002] Linden tree (Tiliatuan) has beautiful tree shape, thick leaves, strong fragrance of flowers, long life span, few pests and diseases, smoke and dust adsorption capacity, strong resistance to harmful gases, and is a world-renowned street tree species. The leaves of linden trees are golden yellow in autumn and have a long leaf-viewing period, so they can be used for urban autumn landscape construction. The linden tree is soft and not easy to deform. It is a high-quality raw material for plywood. In addition, linden flowers can produce a large amount of nectar, which is an important nectar source plant in my country. Its flowers and bracts are raw materials for making scented tea and extracting aromatic oil in Europe. Due to the high economic value of the linden tree itself, the wild ge...

Claims

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Application Information

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IPC IPC(8): A01C1/00A01N47/42A01P21/00
CPCA01C1/00A01N47/42
Inventor 宋跃朋刘鹏陈盼飞张德强
Owner BEIJING FORESTRY UNIVERSITY
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