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Primer combination for detecting genes related to skin moisturizing abilities and application of primer combination

A primer combination and primer set technology, applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of inability to achieve simultaneous detection of SNP sites, low sensitivity, and long time consumption.

Pending Publication Date: 2020-07-07
广州市普森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The research progress of human genetics, molecular biology, genomics and bioinformatics has fully demonstrated that skin moisturizing ability is affected by single nucleotide polymorphism (single nucleotide polymorphism, SNP), and the patent (201810839384.4) discloses a skin-related The gene locus library and its construction method and application, the stratum corneum hydration-related gene ST14, the skin water-holding and moisturizing ability gene AQP3, the keratin production-related gene DAG and the skin natural moisturizing factor production-related gene FLG, a total of 4 genes and 5 bits point, the detection aspect selected by the patent is not fully in line with the purpose of moisturizing gene detection, including other detection aspects, and the detection site cannot cover the detection requirements
The patent (201811356181.6) discloses a method for rapidly detecting skin moisturizing ability genes. By detecting two sites related to moisturizing, the skin moisturizing ability is detected, and high resolution melting curve analysis technology (High Resolution Melting, HRM) is used to analyze The genotype of each SNP locus of the sample to be tested, although the method is simple to operate, it cannot meet the actual needs of simultaneous detection of a large number of SNP loci, it takes a long time, costs a lot, and has low sensitivity

Method used

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  • Primer combination for detecting genes related to skin moisturizing abilities and application of primer combination
  • Primer combination for detecting genes related to skin moisturizing abilities and application of primer combination
  • Primer combination for detecting genes related to skin moisturizing abilities and application of primer combination

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0081] Example 2 Sample detection process

[0082] In order to verify the accuracy and practicability of the multiplex PCR amplification primer set and single base extension primer set design for detecting the SNP site of the skin moisturizing ability gene of the present invention, 30 oral swab samples were collected in this embodiment for detection , the details are as follows:

[0083] 1. Primer design:

[0084] According to the SNP site sequence information, use the design software Assay Design Suite V2.0 of Agena Company to design multiple PCR amplification primers and single-base extension primers for the SNP sites to be tested, and multiplex PCR of 12 SNP sites in 7 genes Amplification primer sets and single base extension primer sets were designed into one reaction (WELL) and validated with UCSC GenomeBioinformatics.

[0085] The result of the quality value of the primer combination of the design software shows that the quality of the primer combination published by t...

Embodiment 3

[0143] Example 3 Primer Comparison

[0144] Using multiplex PCR amplification primer set and single base extension primer set designed by the present invention and ordinary multiplex PCR amplification primer set and single base extension primer set (Table 13) to detect buccal swab DNA samples respectively, sample extraction and detection The specific implementation methods and steps are as above-mentioned Example 1 and Example 2, and the result data are shown in Table 14.

[0145] Table 13 Common Multiplex PCR Amplification Primer and Single Base Extension Primer Sequence

[0146]

[0147]

[0148] The test result data of this comparative experiment shows that the multiplex PCR amplification primer set and the single base extension primer set designed by the present invention are more suitable for the detection of the SNP site of the skin moisturizing ability gene than the ordinary multiplex PCR amplification primer set and the single base extension primer set (Table 14...

Embodiment 4

[0151] Embodiment 4 test result statistics and interpretation

[0152] The detection results of 12 SNP sites of 7 skin moisturizing genes in the 30 oral swab samples that have been detected in Example 3 are counted (Table 15), and the SNP sites are carried out according to the detection result interpretation standard (Table 16) Interpretation of molecular biology genotyping results (Table 17, 18), comprehensively evaluate the genetic information of the subject's skin moisturizing ability, and use it to guide precise skin care management.

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Abstract

The invention relates to the technical field of genotyping, in particular to a primer combination for detecting genes related to skin moisturizing abilities and application of the primer combination.The primer combination includes a multiple-PCR-amplification primer group and a single base extension primer group which are used for detecting 12 SNP sites of 7 skin moisturizing ability genes in thehuman genome, wherein a pair of multiple-PCR-amplification primers and a single base extension primer are designed for each SNP site, and all the sites are designed into one reaction, and can be detected simultaneously. An SNP typing method for efficiently detecting the genes relates to skin moisturizing abilities is established, molecular biology genotyping of multiple SNP sites can be completedat the same time, and the method has good detection sensitivity, high accuracy, low cost and high practicability, can be applied to detection of genetic factors which influence skin moisturizing abilities, and applied to comprehensive evaluation of skin moisturizing abilities, so that development of personalized services in the beauty industry is promoted.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a combination of primers for gene detection of skin moisturizing ability and its application. Background technique [0002] Dry skin can occur at any age, in any season, and under various adverse environments, so moisturizing the skin is particularly important. Moisturizing means that water molecules are firmly locked in the deep layer of the skin, so that the skin is not prone to problems such as tightness, increased dandruff, dryness, relaxation, fine lines, sensitivity, wrinkles, and chapped skin. If the above situation occurs, you can use moisturizing skin care products to prevent skin moisture from evaporating, improve microcirculation, and enhance skin moisture. At this stage, people pay more and more attention to the demand for skin moisturizing, and the types of moisturizing skin care products in skin care products are also emerging in an endless stream. Research sho...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 李文郭之彬张欣鑫廖俊杰
Owner 广州市普森生物科技有限公司
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