Large-scale preparation method and application of polypeptide
A large-scale, synthetic method technology, applied in the field of medicine and biology, can solve problems such as the inability to achieve large-scale synthesis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0110] This example is used to illustrate the method for large-scale synthesis of polypeptides of the present invention.
[0111] This example provides a large-scale synthesis method of E5 polypeptide.
[0112] The amino acid sequence of the E5 polypeptide is: GGRSSFFLLRRIQGCFRRNTVDD
[0113] 1. Synthetic peptide chain
[0114] 1) Link the first amino acid
[0115] Get 400g 2-chlorotriphenyl chloride resin, replace 0.938mmol / g, soak 30 minutes with DCM in the solid-state synthesis reactor, make resin fully swell, use vacuum water pump to drain liquid, add 159g Fmoc-Asp(OtBu)- OH amino acid and 4LDMF, react for 30min.
[0116] Drain the liquid, wash the resin alternately with 4LDMF and DCM three times, and then wash with 4LDMF three times. Drain the liquid. Add 8 L of DCM, 1.5 L of methanol, and 0.5 L of DIEA mixed solution to block the chlorine on the unreacted resin, and react for 10 min. repeat. Wash with 4L DMF and DCM alternately for 3 times, and then wash with 4L D...
Embodiment 2
[0176] This example is used to illustrate the method for large-scale synthesis of polypeptides of the present invention.
[0177] This example provides a method for large-scale synthesis of polypeptides.
[0178] The amino acid sequence of the CD36-6 polypeptide is: FITC-RGVYDVFNGDRNISD
[0179] 1. Synthetic peptide chain
[0180] 1) Link the first amino acid
[0181] Get 400g 2-chlorotriphenyl chloride resin, replace 0.938mmol / g, soak 30 minutes with DCM in the solid-state synthesis reactor, make resin fully swell, use vacuum water pump to drain liquid, add 159g Fmoc-Asp(OtBu)- OH amino acid and 4LDMF, react for 30min.
[0182] Drain the liquid, wash the resin alternately with 4LDMF and DCM three times, and then wash with 4LDMF three times. Drain the liquid. Add 8 L of DCM, 1.5 L of methanol, and 0.5 L of DIEA mixed solution to block the chlorine on the unreacted resin, and react for 10 min. repeat. Wash with 4L DMF and DCM alternately for 3 times, and then wash with 4...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com