A kind of cryopreservation method of buffalo semen

Abstract

The invention relates to a cryopreservation method of buffalo semen, which includes the steps of collecting, diluting, freezing and storing the semen. Firstly, adopting the pseudo-vaginal method to collect the semen, selecting the semen whose color is milky white, filtering and removing the jelly, and checking the quality to meet the standard Preheat the semen to 35°C; dilute the semen after preheating the diluent to 35°C, cool down the diluted semen to 4°C, divide into 0.25mL thin tubes, seal and freeze, the freezing procedure is: temperature Start at 4°C, maintain 4°C for 30 minutes, then drop to -10°C at a rate of 1-5°C / min, then drop from -10°C to -140°C at a rate of 30-40°C / min, and finally -140°C Keep it for 10 minutes, complete the freezing, and store it in liquid nitrogen. The method has simple steps and high practicability, and the thawing activity of the obtained frozen semen is as high as 67.66%, which greatly exceeds the collection requirements of the national livestock gene bank.

Description

technical field [0001] The invention relates to a semen preservation method, in particular to a method for cryopreservation of buffalo semen, and belongs to the technical field of livestock reproduction. Background technique [0002] Shanghai buffalo are native to the suburbs of Shanghai such as Jiading, Baoshan, Fengxian and Chongming. Larger in size, cows weighing 700 to 900 kg and bulls over 1000 kg. The buffalo was originally an important meat and livestock in the southern rice region of my country. According to the "Shanghai Livestock and Poultry Breeds Record" in 1987, with the development of agricultural mechanization and rural urbanization, the value of buffalo has decreased. The number of buffalo in Shanghai has been raised since 1972. After 2000, the number of buffaloes decreased at a rate of 0.13 per year. By 1982, there were only 28,900 buffaloes left, and the number of fine-bred buffaloes dropped sharply. Shanghai buffalo was once reported to be on the verge of...

AI Technical Summary

Problems solved by technology

At present, the production process of bovine frozen semen in China is implemented in accordance with the technical regulations for the production of bovine frozen semen of the People's Republic of China, but the recovery rate of frozen semen sperm is related to factors such as the quality of the original semen, the type of diluent, the freezing temperature and thawing temperature of the semen, among which , the temperature change of semen during the freezing process is one of the important factors affecting the quality of sperm freezing. At present, domestic cattle frozen semen production mostly adopts the freezing procedures provided by freezing equipment manufacturers. Basically, the same freezing procedure is used for various varieties of cattle. In the production of frozen semen, the sperm motility rate after thawing is relatively low

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