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Metabonomics analysis method for garlic bulb components in different growth stages

A technology of growth stage and metabolomics, applied in the field of detection and analysis, can solve the problems of inability to provide guidance for garlic application and inability to intuitively respond to changes, etc.

Pending Publication Date: 2020-06-26
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing detection methods cannot intuitively reflect the changes of various metabolites in different growth stages, and cannot provide guidance for the application of garlic in different growth stages

Method used

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  • Metabonomics analysis method for garlic bulb components in different growth stages
  • Metabonomics analysis method for garlic bulb components in different growth stages
  • Metabonomics analysis method for garlic bulb components in different growth stages

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0019] The preparation process of garlic bulb samples includes: peeling the collected garlic bulbs in different growth stages, crushing after freeze-drying to obtain garlic powder, extracting garlic powder to obtain garlic extract to facilitate the separation and qualitative analysis of metabolites .

[0020] Among them, the collection process of garlic bulb samples at different growth stages included: sampling started when the garlic buds began to develop, sampling once every one week, and ending sampling two weeks after the garlic was normally harvested.

[0021] Preferably, the freeze-drying process is to dry at -80 to -60°C for more than 48 hours, and drying at a lower temperature can fully preserve metabolites, so as to prevent the loss of metabolites and affect the accuracy of analysis. The pulverization process is to pulverize the freeze-dried garlic bulbs to 50-70 meshes, so as to better extract active ingredients.

[0022] Further, the preparation of the garlic extra...

Embodiment 1

[0043] This embodiment provides a method for metabolomics analysis of garlic bulb components in different growth stages, comprising the following steps:

[0044] The garlic variety in this example is Xusuan 917, grown in the experimental field of the Jiangsu Academy of Agricultural Sciences in Xuzhou City, Jiangsu Province (N34°16′45.26″, E117°17′27.29″), sown in October 2018 and harvested in May 2019 receive. Sampling began when the garlic buds began to develop, and was sampled every other week until two weeks after the garlic was normally harvested. The sample collection time is April 18 (week-1), April 25 (week-2), May 2 (week-3), May 9 (week-4), and May 16, 2019. Day (week-5), May 23 (week-6), May 30 (week-7). All samples were collected in 3 replicates, and at least 20 garlic bulbs were collected in each growth stage of each replicate.

[0045] (1) Sample preparation: After the garlic bulbs were peeled, about 300 g of undamaged garlic bulbs were taken from each replicat...

Embodiment 2

[0063] This embodiment provides a method for metabolomics analysis of garlic bulb components in different growth stages, which is the same as the specific steps in Example 1, the only difference being the control of parameters:

[0064] (1) Sample preparation: After the garlic bulbs were peeled, about 300 g of undamaged garlic bulbs were taken from each replicate and freeze-dried at -80°C. After the garlic was freeze-dried, it was crushed with a parallel grinder, passed through a 60-mesh sieve, and stored in a -80°C refrigerator for testing. Weigh 200 mg of freeze-dried garlic powder sample and place it in a 50 mL centrifuge tube, add 14 mL of methanol / water (50 / 25, v / v) solution containing 0.08% formic acid, and vortex for 1 min to fully mix it with the sample; Sonicate at 20°C for 10 minutes, then centrifuge at 8,000 rpm for 3 minutes at 3°C ​​in a low-temperature centrifuge; take 1 mL of the supernatant, pass it through a 0.1 μm organic filter into a 2 mL injection vial, an...

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Abstract

The invention discloses a metabonomics analysis method for garlic bulb components in different growth stages, and relates to the technical field of detection and analysis. The metabonomics analysis method for the garlic bulb components in different growth stages comprises the following steps of: performing chemical component non-targeted metabonomics analysis on garlic bulb samples in different growth stages, processing acquired data to screen out differential metabolites, identifying and confirming the obtained differential metabolites, and analyzing change rules of the differential metabolites in different growth stages. Differential metabolites in garlic bulbs in different growth periods can be quickly and accurately found out, the accumulation rule of the metabolites in the bulbs can be known, and moreover, the garlic bulbs in different growth periods can be selected according to different consumption, processing and other requirements, so that a basis is provided for reasonable consumption and timely harvesting of garlic.

Description

technical field [0001] The invention relates to the technical field of detection and analysis, and in particular to a metabolomics analysis method for garlic bulb components in different growth stages. Background technique [0002] Garlic (Latin name: Allium sativum L.) is one of the most important medicinal and edible plants in the world. It has important physiological functions such as antibacterial, anti-cancer and anti-cancer, prevention and treatment of cardiovascular diseases, and anti-oxidation. Garlic not only contains functional components such as alliin, but also contains rich amino acids, polyphenols, vitamins and other nutrients. Garlic bulbs have edible value throughout the growth process, and their nutritional quality varies greatly in different growth stages, and garlic at different stages may have different application values. However, the existing detection methods cannot intuitively reflect the changes of various metabolites in different growth stages, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8809
Inventor 邱静刘平香钱永忠
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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