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Skin whitening capability gene detection primer combination and application thereof

A primer combination and skin whitening technology, which is applied in the field of skin whitening ability gene detection primer combination, can solve the problems of low specificity, formulating personalized skin care programs, and no detection sites, so as to avoid cross-contamination, slow down skin dullness, The effect of low error rate

Pending Publication Date: 2020-06-23
广州市普森生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent (201910480054.5) discloses a method for rapid detection of hereditary skin whitening ability genes. The invention patent detects two genetic loci related to skin whitening, namely the rs1805007 locus of the MC1R gene and the rs1800414 locus of the OCA2 gene According to the genotypes of these two SNP sites, the skin’s genetic whitening ability can be judged. The detection sites selected by this patent are too few, and only the genes regulating melanin synthesis (MC1R) and tyrosine (melanin precursor) are detected. ) transporter gene (OCA2), while the melanin regulation gene (HERC2), tyrosinase synthesis gene (TYR) and so on are not detected, the coverage of detection sites is not comprehensive enough to truly reflect the innate skin whitening ability of the human body
The patent (201811276285.6) discloses a combination of primers for rapid detection of human skin quality and its application. There are only three related gene loci for detection of skin whitening, but the method is determined by PCR fluorescence quantitative (HRM method). Although this method is simple to operate , but it is impossible to detect a large number of SNP sites at the same time, and the detection time is long and the specificity is low
The patent (201810839262.5) discloses a method for formulating a personalized skin care plan based on the detection of skin color-related genes. There are 22 skin whitening-related genes detected. Although there are many genes detected, they are relatively comprehensive, but some detection genes and skin The whitening ability does not meet, such as calmodulin gene (CaM), loricrin gene (LOR), etc., and only the detection gene is announced without specific detection sites. There is no detection method and detection result description in the examples. Nor does it explain how to develop personalized skin care regimens based on different genetic test results

Method used

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  • Skin whitening capability gene detection primer combination and application thereof
  • Skin whitening capability gene detection primer combination and application thereof
  • Skin whitening capability gene detection primer combination and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] A skin whitening ability gene detection primer set and application thereof, including a multiplex PCR amplification primer set and a single base extension primer set for detecting 12 SNP sites of 10 skin whitening ability genes in the human genome (Table 2). A pair of multiplex PCR amplification primers and a single-base extension primer are designed for each SNP site, and all sites are designed into one reaction for simultaneous detection. The multiple PCR amplification primer set and the single base extension primer set are used in the MassARRAY nucleic acid mass spectrometry analysis system, which can be used for rapid analysis of nucleic acid samples with high sensitivity and high accuracy, and the detection accuracy rate is ≥99.9%. The invention establishes an efficient SNP typing method for comprehensively evaluating skin whitening ability genes, which can complete the molecular biology genotyping of multiple SNP sites at the same time, with good detection sensitiv...

Embodiment 2

[0081] Example 2 Sample Detection

[0082] In order to verify the accuracy and practicability of the design of the multiplex PCR amplification primer set and single base extension primer set for detecting the SNP site of the skin whitening ability gene of the present invention, 30 oral swab samples were collected for detection in this example , the details are as follows:

[0083] 1. Primer design:

[0084] According to the SNP site sequence information, use the design software Assay Design Suite V2.0 of Agena Company to design multiple PCR amplification primers and single base extension primers for the SNP sites to be tested, and multiplex PCR amplification primers for 12 SNP sites of 10 genes Amplification primer sets and single base extension primer sets were designed into one reaction (WELL) and validated with UCSC GenomeBioinformatics.

[0085] The result of the quality value of the primer combination of the design software shows that the quality of the primer combinati...

Embodiment 3

[0144] Example 3 Primer Comparison

[0145] Using multiplex PCR amplification primer set and single base extension primer set designed by the present invention and ordinary multiplex PCR amplification primer set and single base extension primer set (Table 13) to detect buccal swab DNA samples respectively, sample extraction and detection The specific implementation methods and steps are as above-mentioned Example 1 and Example 2, and the result data are shown in Table 14.

[0146] Table 13 Common Multiplex PCR Amplification Primer and Single Base Extension Primer Sequence

[0147]

[0148]

[0149] The test result data of this comparative experiment shows that the multiplex PCR amplification primer set and the single base extension primer set designed by the present invention are more suitable for the detection of the SNP site of the skin whitening ability gene than the ordinary multiplex PCR amplification primer set and the single base extension primer set (Table 14). ...

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Abstract

The invention relates to the technical field of genotyping, in particular to a skin whitening capability gene detection primer combination and an application thereof. The skin whitening capability gene detection primer combination comprises multiplex PCR amplification primer combinations and a single base extension primer combination for detecting 12 SNP sites of 10 skin whitening capability genesof a human genome. Each SNP site is designed with a pair of multiplex PCR amplification primers and a single base extension primer, and all of sites are designed in a reaction to be detected at the same time. According to the SNP typing method for efficiently detecting the skin whitening capability gene, molecular biology genotyping of multiple SNP sites can be completed at the same time, the detection sensitivity is high, the accuracy is high, the cost is low, the practicability is high, the genetic factors influencing skin whitening ability are detected, the comprehensive evaluation of skinwhitening ability is carried out, and the development of individualized services in the beauty industry is promoted.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a skin whitening ability gene detection primer combination and application thereof. Background technique [0002] Heart of beauty in everyone. It is human nature to love beauty. Modern people's pursuit of beauty has reached an unprecedented height. As the saying goes, "one white covers three ugliness". People's pursuit of whitening has never stopped just like human pursuit of light. Skin whitening has already Become an essential part of skin care. According to the "Cosmetics Industry Report" produced by 360 Marketing Institute, among all the cosmetics used by the Chinese population, cosmetics for whitening and freckle accounted for 40.4%, nearly half of the share, and have become the largest category of cosmetics, while acne, acne, Moisturizing, sunscreen and anti-aging products accounted for 19.7%, 8.7%, 7.5% and 6.1% respectively. And compared with 2017, the traffic of wh...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6872C12Q1/686C12N15/11
CPCC12Q1/6883C12Q1/6872C12Q1/686C12Q2600/156C12Q2600/148C12Q2600/16C12Q2537/143
Inventor 李文郭之彬张欣鑫廖俊杰
Owner 广州市普森生物科技有限公司
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