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Hypersensitive cardiac troponin I detection method and kit

A cardiac troponin and detection kit technology, applied in the field of protein detection, can solve the problems of low detection rate, missed detection, insufficient sensitivity and specificity, etc., to achieve large coverage, improve detection rate, and reduce missed detection. effect of risk

Active Publication Date: 2020-06-19
GUANGDONG WESAIL BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these products mostly use antibodies to a certain epitope to detect antigens, and there are generally missed detections and the detection rate of normal people cannot reach ≥ 50%.
Therefore, the performance of current kits generally does not meet the requirements of hypersensitivity, such as low detection rate, insufficient sensitivity and specificity, etc., cannot monitor the development trend of the disease, and cannot meet the requirements of detecting changes in cardiac troponin levels in blood of patients at different times.

Method used

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  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit
  • Hypersensitive cardiac troponin I detection method and kit

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Embodiment Construction

[0062] The present invention will be described in further detail below mainly in conjunction with specific embodiments. The following examples are provided to illustrate embodiments of the invention and are not intended to limit the invention. The invention may optionally include embodiments not shown by way of example.

[0063] 1. Preparation of cardiac troponin I monoclonal antibody

[0064] 1. Immune animals: take BALB / c mice of the same strain as myeloma cells at the age of 8 to 12 weeks, and use cTnI antigen (SEQ ID NO: 1) fully mixed with an equal amount of Freund's complete adjuvant to give 100 μg of antigen The protein / mouse was injected into the intraperitoneal cavity of the mouse, and the cTnI antigen, which was thoroughly mixed with the same amount of Freund's incomplete adjuvant, was injected into the intraperitoneal cavity of the mouse several times to boost the immunization with 100 μg antigenic protein / mouse. After the detection of mouse serum (indirect ELISA ...

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Abstract

The invention relates to a hypersensitive cardiac troponin I detection method and a kit. The kit comprises a first group of antibodies and a second group of antibodies for detecting cardiac troponin Iin a sample from a subject, wherein the antibodies are selected from antibodies specifically binding to the following amino acid fragments: antibody 1: 13th to 29th sites; antibody 2: 27th to 39th sites; antibody 3: the 41st to 49th sites; and an antibody 4: the 85th to 92nd sites. The method and the kit disclosed by the invention are high in sensitivity and simple to operate, and can be used fordynamically monitoring the cardiac troponin I.

Description

technical field [0001] The invention relates to the field of protein detection. Specifically, the present invention relates to a detection method and a kit for hypersensitive cardiac troponin I. Background technique [0002] Elevation of blood cardiac troponin I (cTnI:cardiac troponin I) has very high sensitivity and specificity for the diagnosis of myocardial injury / necrosis (myocardial necrosis). At present, cTnI has become an essential marker for the clinical diagnosis, treatment and monitoring of myocardial infarction. [0003] Cardiac troponin exists in a small amount in normal people, but when myocardial infarction occurs, myocardial cell damage causes cTnI to be released into the blood, no matter whether the ST segment is elevated or not, cTnI will rise sharply, reaching the peak at about 24 hours, and after 7-10 days dropped to normal levels. There is a certain degree of correlation between the cTnI elevation and the degree of myocardial cell necrosis. If the cTn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577C07K16/18
CPCC07K16/18C07K2317/34G01N33/577G01N33/6887G01N33/6893G01N2333/4712G01N2800/324
Inventor 刘丽萍于鸫卢盛萍迟晨黄春连何智丹李斌王凯李文静李然覃素妮
Owner GUANGDONG WESAIL BIOTECH CO LTD
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