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Serum-based rapid brucella vaccine strain infection detection method

A technology of Brucella, detection method, applied in the field of protein fingerprint detection

Active Publication Date: 2020-06-19
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There have been reports on the use of MALDI-TOF MS for the detection of Brucella in blood cultures, but there is no report at home and abroad on the use of MALDI-TOF MS technology to directly detect serum to determine Brucella infection

Method used

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  • Serum-based rapid brucella vaccine strain infection detection method
  • Serum-based rapid brucella vaccine strain infection detection method
  • Serum-based rapid brucella vaccine strain infection detection method

Examples

Experimental program
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Effect test

Embodiment 1

[0034] The determination of embodiment 1 characteristic protein and the construction of standard detection model

[0035] 1. Modeling training sample selection

[0036] The present invention adopts the serum sample of 30 routine healthy people (brucellosis antibody negative), the serum sample that 30 routine Brucella vaccine strains infect, and the vaccine strain of brucellosis has S2 and A19, and all vaccine strain infection samples are passed through popularization. Pathological investigation, cysteine ​​agglutination test confirmed. 20% of the sample size is used for model cross-validation.

[0037] 2. Serum protein sample enrichment: Take out the magnetic bead kit (Bruker MB-WCX kit) from the refrigerator at 4°C, take out a tube of weak cation magnetic bead suspension, turn it upside down manually, and mix the magnetic bead suspension completely for 1 minute; Draw 60 μL of magnetic bead binding buffer (BB) and add it to a 200 μL sample tube, then add 10 μL of magnetic be...

Embodiment 2

[0049] Example 2 Model Classification Ability Detection

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Abstract

The invention provides a serum-based rapid brucella vaccine strain infection detection method, and relates to the technical field of protein fingerprint spectrum detection. The invention provides characteristic protein combination for identifying serum infection of a brucella vaccine strain. The characteristic protein combination is used for constructing a standard detection model of vaccine strain infected brucellosis serum through a GA model algorithm of ClinProTools software, the recognition capability of the constructed model is 100%, and the cross validation value is 99.56%. MALDI-TOF MSmass spectrum data of to-be-detected serum are classified and analyzed by utilizing the standard detection model, and whether the to-be-detected serum is infected by a brucella vaccine strain or not can be accurately judged. The method is suitable for serum detection of high-risk groups infected by Brucella vaccine strains, is high in accuracy, good in repeatability and high in flux, detection of96 samples can be completed within 4 hours, the detection cost is low and the result is reliable, and the method has the broad application prospect.

Description

technical field [0001] The invention relates to the technical field of protein fingerprint detection, in particular to a mass spectrometry model for detecting serum characteristic proteins and a method for identifying whether an asymptomatic person is infected with a Brucella vaccine strain based on the mass spectrometry model. Background technique [0002] Brucellosis (Brucellosis, referred to as brucellosis) is an infectious-allergic infectious disease caused by Brucella bacteria invading the body. Human infection is mainly through contact with infected animals or eating food contaminated by Brucella. After a person suffers from this disease, multiple organs may be damaged, and it is easy to turn into a chronic patient that is difficult to cure. In severe cases, it may become disabled and seriously affect the quality of life. [0003] Animal immunization is the most economical means to prevent and control animal brucellosis. Veterinarians who immunize animals, employees ...

Claims

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Application Information

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IPC IPC(8): G01N27/62G01N33/68G16B5/00G16B40/00
CPCG01N27/62G01N33/6851G16B5/00G16B40/00Y02A50/30
Inventor 姜海肖迪张慧芳张炳华王磊杨文涛李天一赵飞
Owner ICDC CHINA CDC
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