Quantitative detection method for sulfonamide-antibiotic-resistant genes in seawater bathing spot

Abstract

The invention discloses a quantitative detection method for sulfonamide-antibiotic-resistant genes in a seawater bathing spot. The method comprises the steps of subjecting a water sample and depositsof the seawater bathing spot to collecting and low-temperature storage, carrying out DNA extraction and purification on the collected sample, then, amplifying DNA fragments of the antibiotic-resistantgenes, cloning the DNA fragments to a host cell to construct standard plasmids, preparing a fluorescent quantitative PCR standard curve of a target DNA standard substance, and calculating the contentof the antibiotic-resistant genes in the sample according to the PCR standard curve of the standard substance and a CT value of the purified sample. According to the method disclosed by the invention, the steps are simple, the operation is convenient, a sample DNA sequence analyzing step is omitted, and the content of the antibiotic-resistant genes in the sample water and the deposits is obtainedaccording to the PCR standard curve and the CT value of the sample, so that the method is wide in applicable range.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a quantitative detection method for sulfonamide antibiotic resistance genes in bathing beaches. Background technique [0002] With the increasing development of coastal tourism resources and the continuous increase of coastal population, bathing beaches have gradually become one of the important media for the spread of antibiotic resistance genes. Antibiotic resistance genes will be brought into nearshore seaside tourist areas along with rainwater erosion, tides and ocean currents. Through the transfer of genetic material between bacteria species, antibiotic resistance genes may be introduced into human pathogenic bacteria in seawater environment. Because the beach entertainment waters carry a large number of tourists, these antibiotic resistance genes can cause fatal intestinal diseases among swimmers swallowed by seawater, thus greatly increasing the possibility o...

AI Technical Summary

Problems solved by technology

Therefore, the pollution of sulfa resistance genes caused by sulfa antibiotics is becoming more and more serious

However, the research on the distribution of resistance genes in bathing beaches started late in China, and less attention has been paid to them. At present, a unified detection method has not been established to detect antibiotic resistance genes in bathing beaches.

Images