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Lead-resistant bacillus velezensis and separation method and application thereof

A technology of bacillus and isolation method, which is applied in the field of lead-resistant Bacillus velei and its isolation, can solve the problems of side effects of the body, difficulty in reaching the poisoning dose, questionable economy and applicability, etc., and achieve a good lead adsorption rate , the effect of strong adsorption effect

Active Publication Date: 2020-06-19
JIANGSU INST OF POULTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the above-mentioned method still produces the following disadvantages. The use of large doses of chelating agents has side effects on the body, and the supplementation of trace elements and vitamins can relieve high doses of lead. However, it is difficult for lead in feed to reach a very high poisoning amount under normal circumstances. Its economy and applicability are debatable

Method used

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  • Lead-resistant bacillus velezensis and separation method and application thereof
  • Lead-resistant bacillus velezensis and separation method and application thereof
  • Lead-resistant bacillus velezensis and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] This example provides the isolation method and application of lead-resistant Bacillus velesii.

[0041] The isolation method of lead-resistant Velez bacillus comprises the steps:

[0042] (1) Take 1 g of cecal contents into a sterile 15 ml centrifuge tube, then add 10 ml of sterile PBS solution, and shake for 30 min;

[0043] (2) Centrifuge in a centrifuge at a speed of 4000rpm, centrifuge for 10min, and take 0.5ml of the supernatant in a 1ml centrifuge tube;

[0044] (3) Place the supernatant in step (2) in a water bath at 80° C. for 20 minutes;

[0045] (4) Take it out after heating in the water bath, and gradually dilute to 10 with sterile PBS -5 , take 100 μL of dilutions of each level and spread them on the LB solid medium containing Pb 500 mg / L, culture at 37°C for 24 hours, and pick a single colony one;

[0046] (5) Continue to inoculate the single bacterium colony 1 picked out in step (4) on the LB solid medium containing Pb 500mg / L, after continuing to culti...

Embodiment 2

[0051] This example provides the isolation method and application of lead-resistant Bacillus velesii.

[0052] The isolation method of lead-resistant Velez bacillus comprises the steps:

[0053] (1) Take 1 g of cecal contents into a sterile 15 ml centrifuge tube, then add 10 ml of sterile PBS solution, and shake for 30 min;

[0054] (2) Centrifuge in a centrifuge at a speed of 4000rpm, centrifuge for 10min, and take 0.5ml of the supernatant in a 1ml centrifuge tube;

[0055] (3) Place the supernatant in step (2) in a 90° C. water bath for 15 minutes;

[0056] (4) Take it out after heating in the water bath, and gradually dilute to 10 with sterile PBS -5 , take 100 μL of dilutions of each level and spread them on the LB solid medium containing Pb 500mg / L, culture at 37°C for 12 hours, and pick a single colony one;

[0057] (5) Continue to inoculate the single bacterium colony 1 picked out in step (4) on the LB solid medium containing Pb 500mg / L, after continuing to cultivate...

Embodiment 3

[0062] This example provides the isolation method and application of lead-resistant Bacillus velesii.

[0063] The isolation method of lead-resistant Velez bacillus comprises the steps:

[0064] (1) Take 1 g of cecal contents into a sterile 15 ml centrifuge tube, then add 10 ml of sterile PBS solution, and shake for 30 min;

[0065] (2) Centrifuge in a centrifuge at a speed of 4000rpm, centrifuge for 10min, and take 0.5ml of the supernatant in a 1ml centrifuge tube;

[0066] (3) Place the supernatant in step (2) in a water bath at 80° C. for 30 minutes;

[0067] (4) Take it out after heating in the water bath, and gradually dilute to 10 with sterile PBS -5 , take 100 μL of dilutions of each level and spread them on the LB solid medium containing Pb 500 mg / L, culture at 37°C for 36 hours, and pick a single colony one;

[0068] (5) Continue to inoculate the single bacterium colony 1 picked out in step (4) on the LB solid medium containing Pb 500mg / L, after continuing to culti...

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Abstract

The present invention discloses lead-resistant bacillus velezensis and a separation method and an application thereof. The provided lead-resistant bacillus velezensis is deposited in China General Microbiological Culture Collection Center and has deposit number of CGMCC NO.19020. The lead-resistant bacillus velezensis is derived from contents of chicken cecum and screened by a LB solid culture medium containing lead. The bacillus velezensis can survive in the environment with a high concentration of lead, has a relatively strong lead adsorption effect, can be used to remove the lead element inanimal feeds, and can enable animals to grow healthily.

Description

technical field [0001] The invention relates to the field of microorganism isolation methods, in particular to a lead-resistant Bacillus velesii and its isolation method and application. Background technique [0002] Bacillus, a family of bacteria, bacilli or cocci capable of forming spores (endospores). Including Bacillus, Lactobacillus, Clostridium, Desulfoenterobacter, and Bacillus sarcina. They are highly resistant to external harmful factors, widely distributed, and exist in soil, water, air, and animal intestines. Bacillus Velez is widely distributed in nature, has good characteristics of rapid growth and stability, is easy to isolate and cultivate, is harmless to humans and animals, and does not pollute the environment. It has abundant metabolites, broad-spectrum antibacterial activity and strong anti-stress ability, so this bacterium is playing an increasingly important role in many fields such as agriculture, environment and fermentation industry. [0003] Lead i...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A23K10/18A23L5/20A61K35/742A61P31/04C12R1/07
CPCA61K35/742A23K10/18A23L5/28A61P31/04C12N1/02C12N1/20C12N1/205C12R2001/07
Inventor 陈大伟高玉时蒲俊华刘茵茵马丽娜陆俊贤唐修君葛庆联贾晓旭
Owner JIANGSU INST OF POULTRY SCI
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