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Radix astragali seedling culture method of radix astragali high in astragaloside IV content

A technology of astragaloside IV content, applied in the field of plant cultivation, can solve the problems of "pesticide residue" exceeding the standard, soil micro-ecological damage, quality degradation, etc., and achieve the effects of improving permeability, improving survival rate, and preventing pollution

Inactive Publication Date: 2020-06-19
内蒙古自治区生物技术研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to production needs, "continuous stubble" planting is usually used, so a large amount of chemical fertilizers and pesticides have to be used, resulting in serious damage to soil micro-ecology, quality degradation, and excessive "pesticide residues"

Method used

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  • Radix astragali seedling culture method of radix astragali high in astragaloside IV content
  • Radix astragali seedling culture method of radix astragali high in astragaloside IV content

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for raising astragalus seedlings with high astragaloside IV content includes the following steps:

[0027] (1) Take an appropriate amount of Mongolian Astragalus seeds, add 4 times the weight of water, add an appropriate amount of sodium hydroxide to adjust the pH to 12, and treat at room temperature for 60 minutes, then pour out the water and leave the seeds;

[0028] (2) Add 70% ethanol solution to the Astragalus seeds in step (1), ultrasonic treatment for 40 seconds; wash with water 5 times;

[0029] (3) Add 4 times the weight of 8% sodium hypochlorite solution to the astragalus seeds in step (2) and soak for 15 minutes, wash them with water 5 times and then drain;

[0030] (4) Put the Astragalus seeds in step (3) into the first MS medium for culture, the culture temperature is 20° C., the humidity is 60%, the culture is 12 days for germination, and the first MS medium is replaced at an interval of 4 days;

[0031] Each liter of the first MS medium contains the follow...

Embodiment 2

[0035] A method for raising astragalus seedlings with high astragaloside IV content includes the following steps:

[0036] (1) Take an appropriate amount of Astragalus membranaceus seeds, add 3 times the weight of water, add an appropriate amount of potassium hydroxide to adjust the pH to 11, and treat at room temperature for 50 minutes, then pour the water away and leave the seeds;

[0037] (2) Add a 70% ethanol solution to the Astragalus seeds in step (1), and ultrasonic treatment for 60 seconds; wash with water 5 times;

[0038] (3) Add 4 times the weight of 1% mercury liter solution to the astragalus seeds in step (2) and soak for 15 minutes, wash them with water 5 times and then drain;

[0039] (4) Put the Astragalus seeds in step (3) into the first MS medium for culture, the culture temperature is 20° C., the humidity is 50%, the culture is 12 days for germination, and the first MS medium is replaced at an interval of 4 days;

[0040] Each liter of the first MS medium contains the...

Embodiment 3

[0044] A method for raising astragalus seedlings with high astragaloside IV content includes the following steps:

[0045] (1) Take an appropriate amount of Mongolian Astragalus seeds, add 3 times the weight of water, add an appropriate amount of sodium hydroxide to adjust the pH to 10, and treat at room temperature for 40 minutes, then pour out the water and leave the seeds;

[0046] (2) Add a 60% ethanol solution to the Astragalus seeds in step (1), ultrasonic treatment for 40 seconds; wash with water 4 times;

[0047] (3) Add 4 times the weight of 8% sodium hypochlorite solution to the Astragalus seeds in step (2) and soak for 10 minutes, wash with water 4 times and then drain;

[0048] (4) Put the Astragalus seeds in step (3) into the first MS medium for culture, the culture temperature is 25° C., the humidity is 55%, the culture is 8 days for germination, and the first MS medium is replaced at an interval of 4 days;

[0049] Each liter of the first MS medium contains the following ...

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Abstract

The invention provides a radix astragali seedling culture method of radix astragali high in astragaloside IV content. Radix astragali seeds are subjected to soaking in an alkaline solution, soaking inhigh-concentration ethanol and ultrasonic treatment, so that the germinating rate of the radix astragali seeds can be increased, and the vitality of seedlings is high; through applying 5-8% of a sodium hypochlorite solution or 1% of a mercuric chloride solution, selecting bacteria-free water, and performing sterilization treatment on culture mediums, sterile growing environment is provided, and the survival rate of the seedlings is greatly increased; and besides, through applying different MS culture mediums at different stages of radix astragali seed germination and radix astragali seedlinggrowth and particularly applying N elements and trace organic matter (myo-inositol) at the seedling growth stage, the content of the astragaloside IV is greatly increased.

Description

Technical field [0001] The invention relates to a method for raising seedlings of plants, in particular to a method for raising seedlings of astragalus with high astragaloside IV content, and belongs to the technical field of plant cultivation. Background technique [0002] Astragalus is a perennial herb of the leguminous family, and its root is used as medicine. The main pharmacological effects of Astragalus include tonifying qi and strengthening the surface, diuresis, supporting toxins and purging pus, and promoting muscle growth. It mainly treats shortness of breath, prostration, palpitations, spontaneous sweating, physical weakness and swelling, chronic nephritis, dehydration, chronic diarrhea, qi deficiency, and insufficient qi and blood. , Zhongqi depression, has antibacterial and anti-inflammatory, antihypertensive, diuretic, detoxification and choleretic effects. It is a commonly used Chinese herbal medicine. The domestic market has great demand for Astragalus, which is ...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/00A01C1/08A01G24/10A01G24/30C05G5/23C05G1/00
CPCA01C1/00A01C1/08A01G31/00A01G24/10A01G24/30C05B7/00
Inventor 白朕卿张秀娟随洋吴佳文田雨王睿陈启渊焦巍
Owner 内蒙古自治区生物技术研究院
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