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Method for analyzing double cells in single-cell transcriptome data

A single-cell, double-cell technology, applied in the field of double-cell analysis, can solve the problems of cumbersome steps, high knowledge background, false positives and false negatives, and achieve the effect of improving efficiency

Active Publication Date: 2020-06-16
SINGLERON NANJING BIOTECHNOLOGIES LTD
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Problems solved by technology

The second method relies on the accumulation of existing biological knowledge. Through the method of manual annotation, the double cells formed by the mixture of cell types with known markers are removed, but the intermediate state of cell type conversion may be removed by mistake.
Therefore, the second method requires extremely high knowledge background, cumbersome steps, and high false positives and false negatives

Method used

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  • Method for analyzing double cells in single-cell transcriptome data
  • Method for analyzing double cells in single-cell transcriptome data
  • Method for analyzing double cells in single-cell transcriptome data

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Embodiment 1

[0027] This embodiment discloses a method for automatically identifying double cells and splitting the cell expression profiles in the double cells. The principle is to carry out identification and analysis of double cells based on computer simulation, which mainly includes the following four processes:

[0028] Synthetic Cells: Synthesize cell expression profiles based on experimental data through computer simulations. The experimental data is the detected cell data. Since the proportion of double cells in the experimental data is very low, most of them are single cells, so most of the synthetic cells will be obtained by superimposing two random source single cells in the detected single cell data. into, that is, the formation of synthetic twin cells.

[0029] There are two types of twin cells, homogeneous twin cells are formed by mixing 2 cells belonging to the same cell type, and heterogeneous twin cells are formed by mixing 2 cell types of different kinds. Therefore, the ...

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Abstract

The invention discloses a method for analyzing double cells in single cell transcriptome data, and belongs to the technical field of single cell transcriptome sequencing. Through computer simulation,a cell expression profile is synthesized on the basis of experimental data, and double cells in the experimental data are identified by identifying and synthesizing heterogeneous double cells. The method does not depend on the existing knowledge of cell types and does not depend on the hypothesis of the total expression quantity of the cells, and the full-automatic double-cell identification can be realized on the basis of computer simulation.

Description

technical field [0001] The invention belongs to the technical field of single-cell transcriptome sequencing, and in particular the invention relates to a method for analyzing double cells in single-cell transcriptome data. Background technique [0002] Single-cell transcriptome sequencing technology (scRNA-Seq) has developed rapidly in recent years. Compared with conventional transcriptome sequencing, this technology can improve the resolution of gene expression from the average level of the population to the level of a single cell (specifically, See literature 1, 2). In particular, various Massively Parallel Single Cell Sequencing technologies (Massively Parallel Single Cell Sequencing) based on microfluidic technology that have just emerged since 2015 (see literature 3-5 for details) can separate a large number of cells into single cells and give each A single cell is added with a molecular tag and then jointly amplified and sequenced to build a library, avoiding the tedi...

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Application Information

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IPC IPC(8): G16B40/00G16B30/00
Inventor 范珏
Owner SINGLERON NANJING BIOTECHNOLOGIES LTD
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