Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A saline-alkali-resistant molecular marker c72 of Portunus trituberculatus and its application

A technology of Portunus trituberculatus and molecular markers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems of young crabs' feeding rate, metamorphosis rate and survival rate decline, lack of molecular marker assistance Breeding marks, hindering the development and utilization of water resources and other issues, to achieve the effect of promoting healthy reproduction, increasing feeding rate, and increasing the number

Active Publication Date: 2021-08-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of such water resources to a large extent.
The physiology and biochemistry of Portunus trituberculatus will be affected to a certain extent under the stimulation of high salinity, and high salinity will reduce the feeding rate, metamorphosis rate and survival rate of young crabs
However, the trait of salt-alkaline tolerance has obvious low heritability characteristics, and the genetic progress through traditional breeding methods is slow. It is urgent to use advanced molecular marker-assisted breeding technology to speed up the breeding process, and the identification and innovative application of salt-alkali tolerance molecular markers It is the necessary prerequisite and way to carry out molecular marker assisted breeding
[0003] At present, there are few studies on the development of molecular markers for salt-alkali tolerance in Portunus trituberculatus, and there is a lack of markers that can be applied to molecular marker-assisted breeding in the industry

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A saline-alkali-resistant molecular marker c72 of Portunus trituberculatus and its application
  • A saline-alkali-resistant molecular marker c72 of Portunus trituberculatus and its application
  • A saline-alkali-resistant molecular marker c72 of Portunus trituberculatus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Screening of candidate molecular markers related to saline-alkaline tolerance

[0026] 1. Sequencing data filtering and comparison

[0027] The DNA extraction was carried out using the kit of Quanshijin Company, and the DNA extraction was carried out by using the principle of specific adsorption of DNA on the silica gel membrane spin column. First, put about 30mg of tissue samples into a sterile 1.5ml centrifuge tube, add 200μl LysisBuffer 8 (LB8) and 20μl RNaseA (10mg / ml), shake for about 10s, incubate at room temperature for 2min, add 20μl Proteinase K (20mg / ml), fully shake and mix, incubate at 55°C until completely lysed, add 1.5 times the volume of Binding Buffer 8 (BB8), mix well and add to the spin column, in a high-speed low-temperature refrigerated centrifuge (Eppendorf5804R) Centrifuge at 12000rpm for 30s, and discard the waste liquid. Then add 500μl of Clean Buffer8 (CB8), centrifuge at 12000rpm for 30s, discard the waste solution (repeat once), then add...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a saline-alkali-resistant molecular marker C72 of Portunus trituberculatus and its application. The nucleotide sequence of the molecular marker C72 is shown in SEQ ID No.1, and the nucleotide sequence of the primer pair for detecting the molecular marker C72 is shown in SEQ ID No.2 and SEQ ID No.3 . The molecular marker C72 is a SNP marker, and its saline-alkali tolerance genotype is CT genotype. The molecular marker C72 provided by the present invention can not be limited by the growth stage of Portunus trituberculatus, and can obviously speed up the breeding process of Portunus trituberculatus and quickly breed crab species with excellent characteristics of tolerance to salt and alkali, and use the molecular marker C72 The detection of the salt-alkali tolerance of Portunus trituberculatus is accurate, reliable and easy to operate, which is beneficial to the healthy breeding and development of Portunus trituberculatus, so it has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers of aquatic animals, and in particular relates to a saline-alkali-resistant molecular marker C72 of Portunus trituberculatus and its application. Background technique [0002] Swimming crab (Portunus trituberculatus) belongs to Crustacea, Decapod order, Portunidae, commonly known as swimming crab, is an important large-scale marine economic crab in my country. Swimming crab meat is delicious and nutritious, and is deeply loved by consumers. Saline-alkaline waters are a worldwide low-yield water resource. In China alone, there are 45.87 million hm2 of saline-alkaline waters. Due to the characteristics of high salinity, high alkalinity, high pH and complex ion composition, conventional aquatic animals cannot Among them, normal survival and reproduction hinder the development and utilization of this type of water resources to a large extent. The physiology and biochemistry of Portunus ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张文金玲吕建建任宪云刘萍李健
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com