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Fluorescent gold nano-cluster as well as preparation method and application thereof

A fluorescent gold nanometer and fluorescent technology, applied in the field of fluorescent gold nanoclusters and its preparation, can solve the problems of narrow application range, cumbersome operation, and poor specificity, and achieve the effects of strong sensitivity, high detection efficiency, and high accuracy

Active Publication Date: 2020-06-09
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional chloramphenicol residue detection techniques, including microbiological methods, radioimmunoassays, chromatography and enzyme immunoassays, have problems such as poor specificity, poor sensitivity, narrow application range, expensive instruments, and cumbersome operations.

Method used

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  • Fluorescent gold nano-cluster as well as preparation method and application thereof
  • Fluorescent gold nano-cluster as well as preparation method and application thereof
  • Fluorescent gold nano-cluster as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Preparation of Xylanase-Au NCs

[0040] Take xylanase and add it into ultrapure water to prepare 0.25mM xylanase solution, and prepare 25mM HAuCl 4 The aqueous solution is added to the above-mentioned xylanase solution, so that the xylanase and HAuCl 4 The molar mass ratio is 1:1. After fully mixing with a vortex for 5 minutes, add an aqueous solution of 1M NaOH to make the pH of the mixed solution 12, and then mix again with a vortex for 5 minutes. The Eppendorf tube was placed in a 37°C water bath for 12 hours in the dark to obtain a gold nanocluster polymer with a concentration of 0.16mM, centrifuged at 5,000rpm for 10min, and the supernatant was stored in a 4°C refrigerator in the dark for future use.

[0041] The transmission electron microscope picture (TEM) of present embodiment gold nanocluster is as figure 1 shown, from figure 1 It can be seen that the size of gold nanoclusters is uniform and dispersed, and the particle size is less than 5nm; ...

Embodiment 2

[0046] Embodiment 2: Preparation of Xylanase-Au NCs

[0047] Take xylanase and add it into ultrapure water to prepare 1mM xylanase solution, and prepare 25mM HAuCl 4 The aqueous solution is added to the above-mentioned xylanase solution, so that the xylanase and HAuCl 4 The molar mass ratio is 0.8:1. After fully mixing with a vortex for 5 minutes, add 1M NaOH solution to make the pH of the mixed solution 10, and then mix with a vortex for 5 minutes again. The Eppendorf tube was placed in a water bath at 37°C for 12 hours in the dark to obtain polymers of gold nanoclusters, centrifuged at 5,000 rpm for 10 minutes, and the supernatant was stored in a refrigerator at 4°C in the dark for future use.

Embodiment 3

[0048] Embodiment 3: Preparation of Xylanase-Au NCs

[0049] Take xylanase and add it into ultrapure water to prepare 1mM xylanase solution, and prepare 25mM HAuCl 4 Add the aqueous solution of xylanase to the above xylanase solution, so that the xylanase and HAuCl 4 The molar mass ratio is 1.5:1. After fully mixing with a vortex for 5 minutes, add 1M NaOH solution to make the pH of the mixed solution 12, and then mix again with a vortex for 5 minutes. The Eppendorf tube was placed in a water bath at 30°C for 24 hours in the dark to obtain polymers of gold nanoclusters, centrifuged at 5,000 rpm for 10 minutes, and the supernatant was stored in a refrigerator at 4°C in the dark for future use.

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Abstract

The invention provides a gold nano-cluster as well as a preparation method and an application thereof. The preparation method comprises the following steps: mixing a xylanase solution with an HAuCl4 solution, and sufficiently and uniformly mixing for 5-10 minutes by using a vortex device; adding a NaOH solution to adjust the pH value to be 8-13, and uniformly mixing for 5-10 minutes again by usingthe vortex device; and reacting in a water bath kettle in a dark place for 6-24 hours to obtain the product. The gold nanocluster is sensitive to Hg<2+> response, a detection system is easy and convenient to operate, controllable and short in consumed time, and the gold nanocluster can be applied to detection of the content of Hg<2+> in environmental water samples and soil samples. Meanwhile, theinvention also provides a method for detecting chloramphenicol by using a fluorescence method, and the gold nano-cluster fluorescent probe is combined with a standard curve, so that the detection process is simple, convenient and quick, the detection cost is low, and the method can be used for detecting the content of chloramphenicol in milk and meat. The method has the advantages of low detection limit, wide detection range, high sensitivity and the like, and can provide a new thought for detecting the content of chloramphenicol in animal food.

Description

technical field [0001] The invention belongs to the field of nano-cluster preparation, and in particular relates to a fluorescent gold nano-cluster and its preparation method and application. Background technique [0002] Xylan is a poly-five-carbon sugar that is the main hemicellulose component in plant cells. Xylanase (Xylanase) can be produced by bacteria, actinomycetes, fungi and other microorganisms, mainly by β-1,4-D-endo-xylanase and β-1,4-D-endo-xylanase Glycosidase composition. Xylanase is a specific compound hydrolase system that can degrade xylan into xylooligosaccharides and xylose. With the continuous development and progress of biotechnology, xylanase is used in different industrial processes, including as an additive to wheat flour to improve the quality of color and aroma of flour products, combining pectinase and cellulase to clarify fruit juice and wine, As a food additive in the feed industry, etc. In addition, xylanases also have good applications in ...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/58B82Y20/00B82Y40/00G01N21/64
CPCC09K11/025C09K11/58B82Y20/00B82Y40/00G01N21/6428G01N2021/6432Y02A50/30
Inventor 王宝娟常欣吉成均李全发吴爽陈杰吴雨芬
Owner ANHUI NORMAL UNIV
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