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Test strip and method for detecting profenofos

A technology for profenofos and test strips, which is applied in the field of test strips for detecting profenofos, can solve the problems that it is difficult to meet the rapid detection of a large number of samples and on-site samples, the processing is cumbersome and time-consuming, and the detection cost is high, and the detection time is short, and the detection cost is high. Easy to promote and use, high sensitivity

Active Publication Date: 2020-06-05
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods must be operated under laboratory conditions. The sample pretreatment is cumbersome and time-consuming, and expensive instruments and equipment are required. The detection cost is high, time-consuming, and the operation is complicated. There are great limitations in the actual application process, and it is difficult to meet The need for rapid detection of a large number of samples and on-site samples

Method used

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  • Test strip and method for detecting profenofos
  • Test strip and method for detecting profenofos
  • Test strip and method for detecting profenofos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of test strips for profenofos detection

[0041] The preparation method of the test strip mainly includes the following steps:

[0042] 1) Preparation of a conjugate release pad sprayed with profenofos monoclonal antibody-colloidal gold marker;

[0043] 2) Prepare a reaction membrane with a test line coated with profenofos hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody;

[0044] 3) Assemble the prepared combination release pad, reaction membrane, sample absorbent pad, water absorbent pad and bottom plate prepared in 1) and 2) into test strips.

[0045] The following is a detailed description step by step:

[0046] 1. Synthesis of profenofos hapten (see attached for synthetic route figure 1 )

[0047] 1) Take 1.67g of phosphorus trichloride, add 100mL of carbon tetrachloride to dissolve, add ice bath, cool to 0~5℃, add 1.8g of butanethiol, add 0.78g of sodium bicarbonate, continue to stir for 2h, stop the ...

Embodiment 2

[0081] Example 2 Detection of profenofos in vegetables and fruits

[0082] 1. Sample pretreatment

[0083] Before testing, remove the sample and cut it into smaller pieces or small pieces of less than 1cm; weigh (1.00±0.05)g of the cut sample into a 10mL polystyrene centrifuge tube, add 6mL phosphate buffer, and close the lid , Manually shake for 30s, let it stand for 1 min, and take the supernatant as the sample solution to be tested.

[0084] 2. Test with test strips

[0085] Use a micropipette to draw 80 μL of the sample solution to be tested and drop it vertically into the sample hole; the liquid flow starts timing and reacts for 10 minutes to determine the result.

[0086] 3. Analyze the test results

[0087] Negative (-): T-line color development is deeper than C-line color development or consistent with C-line color development, indicating that the profenofos concentration in the sample is below the detection limit, such as image 3 a, 3b.

[0088] Positive (+): T-line is lighter t...

Embodiment 3

[0090] Example 3 Sample detection example

[0091] 1. Detection limit test

[0092] Take blank samples of cauliflower, kale, radish leaves, common cabbage, and oranges, add profenofos to the final concentrations of 0.05mg / kg, 0.1mg / kg, 0.2mg / kg, and take test strips for testing. The measurement is repeated three times for each sample.

[0093] When testing broccoli, kale, radish leaves, common cabbage and orange samples with test strips, when there is no profenofos and its concentration is 0.05mg / kg, the test strip shows T line color ratio C line The color is deep or consistent with the color of the C line, which is negative; when the profenofos concentration is 0.1mg / kg, 0.2mg / kg, the test strip shows that the color of the T line is lighter than the color of the C line. The T line does not show color and is positive, indicating that the detection limit of profenofos in vegetables and fruits is 0.1mg / kg.

[0094] 2. False positive rate and false negative rate test

[0095] Take sampl...

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Abstract

The invention discloses a test strip and a method for detecting profenofos. The test strip comprises a sample absorption pad, a conjugate release pad, a reaction film, a water absorption pad and a bottom plate. The reaction film is provided with a detection line coated with a profenofos hapten-carrier protein conjugate and a quality control line coated with a goat anti-mouse anti-antibody, and theconjugate release pad is sprayed with a profenofos monoclonal antibody-colloidal gold marker. The invention also provides a method for detecting profenofos in vegetable and fruit samples by using thetest strip. The test strip provided by the invention has the advantages of simplicity in operation, high sensitivity, high detection speed, low cost, suitability for large-batch sample screening andthe like, and can meet the requirements of food supervision departments in China for carrying out on-site monitoring and detection work.

Description

Technical field [0001] The invention relates to a test strip and a method for detecting profenofos, in particular to a colloidal gold test strip for detecting profenofos, which is particularly suitable for the detection of profenofos residues in vegetables and fruits. Background technique [0002] Profenofos is a moderately toxic non-systemic broad-spectrum organophosphorus insecticide with contact and stomach toxicity, and has a good control effect on pests on crops. But with the increasing usage, the pollution caused by its use on vegetables cannot be ignored. Although a small amount of profenofos residues will not cause immediate and direct harm to the human body, long-term consumption of such contaminated vegetables and fruits can cause cancer, infertility, endocrine disorders and other diseases, and can also harm the nervous center. Convulsions to death. my country's national standard GB 2763-2019 "National Food Safety Standard Maximum Residue Limits of Pesticides in Foods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/53
CPCG01N33/577G01N33/558G01N33/5308
Inventor 吴小胜何方洋凡静静宋灏张珊珊杨海涛郑哲哲
Owner BEIJING KWINBON BIOTECH
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