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Botrytis cinerea inhibitor and application thereof

A technology of botrytis cinerea and inhibitor, applied in the field of botrytis cinerea inhibitors, can solve problems such as pesticide toxicity, residue, environmental pollution and the like

Pending Publication Date: 2020-06-05
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of Botrytis cinerea mainly depends on chemical agents, but there are problems such as pesticide toxicity, residue, and environmental pollution.
Long-term use of chemicals has also led to the emergence of resistant Botrytis cinerea in fields

Method used

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  • Botrytis cinerea inhibitor and application thereof
  • Botrytis cinerea inhibitor and application thereof
  • Botrytis cinerea inhibitor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. The preparation of capsaicin:

[0021] Preparation of capsaicin mother liquor: mix capsaicin (80.0mg) and methanol (2mL) to form a capsaicin solution preparation, store at 4°C at low temperature.

[0022] 2. Select the highly pathogenic gray mold bacterial classification (Botrytis cinerea) to test, and the propagation condition of this bacterial classification is as follows:

[0023] 1) Preparation of V8 medium: V8 (360 mL), calcium carbonate (2.0 g), agar (20.0 g). Mix the above components and add distilled water to form a 1000mL solution preparation. After the solution preparation is subjected to high temperature and high pressure (121°C, 15 minutes), it is placed at 4°C for use;

[0024] 2) Preparation of V8 liquor: maltose (8.0g), peptone (2.0g). Mix the above components and add distilled water to form a 200mL solution preparation. After the solution preparation is subjected to high temperature and high pressure (121°C, 15 minutes), it is placed at 4°C for use;...

Embodiment 2

[0033] Pepper 'Hangjiao No. 12' was planted in an artificial climate chamber (12h light / 12h dark, day and night temperature 25°C / 20°C), and when the pepper plants grew to 5 leaves and 1 heart, 3 plants were sprayed with sterile water (control) , 3 plants were sprayed with capsaicin (processing, 80 μg / mL), while the pepper plants of the treatment and contrast were sprayed with Botrytis cinerea 30mL (spore concentration was 1×10 5 unit / mL), observed under high humidity environment. After 3 days the results are as follows image 3 As shown, the three tomato plants in the control group all had gray mold symptoms, while the peppers in the treatment group grew normally. It shows that capsaicin effectively inhibits the activity of Botrytis cinerea in the substrate, thus delaying or even preventing the onset of pepper plants.

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Abstract

The invention provides a botrytis cinerea inhibitor and application thereof, and relates to the technical field of bio-pesticides. A methanol solution of capsaicine is used as an inhibitor for inhibiting the activity of botrytis cinerea. The invention proves that capsaicine with different concentrations has an inhibition effect on Botrytis cinerea and has concentration dependence, the higher the capsaicine concentration is, the more obvious the inhibition effect is; and capsaicine is sprayed to delay or even prevent the morbidity of pepper plants.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to an inhibitor of botrytis cinerea and its application. Background technique [0002] Botrytis cinerea is a necrotrophic pathogenic microorganism with a wide host range, which can cause disease in more than 200 crops, including pepper, tomato, strawberry and other important economic crops; the fungus produces a variety of phytotoxins and Cell wall hydrolytic enzymes kill plant cells to obtain nutrients and sclerotia survive for a long time on plant residues, which not only harm the stems, leaves and fruits on the plants, but also endanger the fruits stored after harvest, seriously affect the yield and quality and are difficult to control. Listed as one of the top ten plant fungal diseases. At present, the control of Botrytis cinerea mainly relies on chemical agents, but there are problems such as pesticide toxicity, residue, and environmental pollution. ...

Claims

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Application Information

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IPC IPC(8): A01N37/18A01P3/00
CPCA01N37/18
Inventor 程远周国治毛奇姚祝平万红建阮美颖王荣青叶青静
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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