Purity identifying method of weak photosensitivity hybrid rice seeds

Abstract

The invention belongs to the technical field of seed purity detection, and discloses a purity identifying method of weak photosensitivity hybrid rice seeds. The method comprises the steps of for the hybrid rice seeds of which restoring line and a sterile line are both a temperature-sensing type, and F1 is weak in photosensitivity, performing single-plant planting on the seeds in the early season or the late season under the condition that the same type of restoring lines, weak photosensitivity sterile lines and restoring lines do not exist in an isolation region and parents hybrid plant typesin the production process of the seeds, according to the earing condition of the weak photosensitivity rice plants at the planting region, counting the number of hybrid plants, and through combinationof the capacity of samples, calculating the seed purity. Based on a rice field small-region planting identifying method, the purity identifying period of the weak photosensitivity hybrid rice seeds produced under special condition is shortened to half a year from one year, the identification time of the field small-region planting purity identifying method of the weak photosensitivity hybrid riceseeds is prolonged to the early season and the later season from the later season, and the identifying location is extended to global regions where rice is suitable for growth from South China rice regions and corresponding appropriate regions.

Description

technical field [0001] The invention belongs to the technical field of seed purity detection, and in particular relates to a method for identifying the purity of weak light-sensitive hybrid rice seeds. Background technique [0002] At present, seed purity is the core indicator of hybrid rice seed quality, the main standard for seed quality grading, and the key basis for companies to purchase and sell seeds. It has always been closely watched by seed management departments, seed companies and farmers. [0003] At present, the methods used in the identification of the purity of hybrid rice seeds mainly include: identification of seed grain morphology, identification of esterase isozymes, identification of planting in field plots, and identification of DNA molecular markers. (1) The identification of seed grain morphology is based on the appearance traits such as seed shape, type, color, size, seedling sheath color, leaf color, leaf shape, etc., to judge the method for the diff...

AI Technical Summary

Problems solved by technology

(1) The identification of seed grain morphology is based on the appearance traits such as seed shape, type, color, size, seedling sheath color, leaf color, leaf shape, etc., to judge the method for the difference between the appearance of the seed and the target seed; different hybrid rice varieties seeds, although The gene polymorphism is large, but due to the limitation of the environment affecting genotype expression or manual observation, the identification accuracy of grain shape difference is limited

(2) The identification of esterase isozymes is based on the specific isozymes or proteins based on biochemical genetics to distinguish and identify the biochemical genetic differences between seeds and target seeds; the esterase isozyme method is to identify different varieties The difference between gene expression product proteins, polymorphism is not rich enough, and there is tissue and organ specificity, the same tissue and organ protein difference is not big, so that the selection of materials is strictly limited, thus limiting its application

Moreover, since the plant phenotype is determined by the genotype and the environment, the expression of plant traits in the field planting identification process is easily affected by the environment, which cannot fully meet the needs of production and management.

(4) DNA molecular marker identification is based on biological DNA restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), microsatellite DNA (SSR, STR, SSLP) and other DNA molecular differences to judge the difference between seeds and target seeds; the accuracy of DNA molecular marker identification is limited by the size of the sample size, in order to reduce the identification error, the larger the sample size, the more accurate the identification, but The amount of corresponding pharmaceutical reagents will also be doubled, which doubles the identification cost and increases the identification time.

[0005] (1) There are limited differences in the grain morphology of different hybrid rice varieties in the existing identification methods of seed grain morphology

[0006] (2) Existing methods for identification of esterase isoenzymes have differences in the identification of different types of gene expression product proteins, polymorphisms are not abundant enough, and have tissue and organ specificity, so that the materials are strictly limited, thus limiting its application

[0007] (3) The existing planting identification methods for field plots are too long, the expression of plant traits is easily affected by the environment, and cannot fully meet the needs of production and management. Generally, the planting identification cycle of temperature-sensitive rice field plots is half a year, and the weak-sensitivity hybridization The planting identification cycle of paddy field plots is one year

[0008] (4) The identification accuracy of existing DNA molecular marker identification methods is limited by the size of the sample. The larger the sample size, the more accurate the identification, but the corresponding doubled the identification cost

[0010] (1) In the identification method of seed grain morphology, the grain morphology is the inherent trait of the variety, and the difference between the variety to be identified and its similar varieties is limited. The selection of varieties should first meet the requirements of traits such as yield increase, stress resistance, and eating quality. The need for purity testing is not necessary to carry out variety selection. The selected varieties have low practical value and are not widely used. This problem is difficult to solve

[0011] (2) In the esterase isoenzyme identification method, protein is an inherent product of gene expression. Like the identification of grain morphology, it is not meaningful to work on protein expression to meet the purity identification requirements, and this problem is difficult to solve

[0012] (3) Field plot planting identification method is currently one of the most accurate and applicable seed purity identification methods. It is the final accurate identification method selected when errors occur in the results of other purity identification methods. It is used by agricultural industry authorities and quality identification agencies. However, in order to fully express the characters that appear in the whole process of rice growth, it is necessary to make each type of rice grow for one cycle, which is difficult to solve.

[0013] (4) In the identification method of DNA molecular markers, increasing the sampling frequency, sample size and number of test samples can properly solve the problem of accuracy. The cost of DNA molecular marker detection is relatively high, and large-scale seed production requires a larger number of test samples. Once the seeds with inaccurate results are sold, if there is a quality problem, it will be a fatal blow to the seed enterprise

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