Photodynamic inhibition of microbial pathogens in plants

A technology of microorganisms, pathogens, applied in the fields of chemicals for biological control, plant growth regulators, botanical equipment and methods, etc.

Pending Publication Date: 2020-05-22
SUNCOR ENERGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing photodynamic suppression techniques and applications suffer from various disadvantages

Method used

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  • Photodynamic inhibition of microbial pathogens in plants
  • Photodynamic inhibition of microbial pathogens in plants
  • Photodynamic inhibition of microbial pathogens in plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0109] In this example, the application of a porphyrin photosensitizer compound and a chelating agent inhibits the growth of the bacterium (Erwinia amylovora) that causes Fire Blight by using photodynamic inhibition. In particular, magnesium chlorophyllin and disodium EDTA were used and their combination was shown to arrest the growth of Erwinia amylovora.

[0110] Erwinia amylovora grows in liquid media. Samples were incubated with 100 μM magnesium chlorophyllin (with or without 5 mM EDTA disodium) for 30 min and then washed with 395 nm (energy flow of 26.6 J cm -2 ) for 30 minutes of irradiation. Count the CFU of bacteria on the plate. The table below summarizes the results:

[0111] Table 1: Results of relative inactivation of bacteria after PDI treatment

[0112] deal with Relative inactivation, CFU Untreated control - in the dark 1 Untreated control - under light 0.9 Magnesium chlorophyllin (100 μM), EDTA-free (5 mM), in light 1.9 Mag...

Embodiment 2

[0115] In this example, the application of a porphyrin photosensitizer compound and a chelating agent inhibits the growth of the fungal pathogen Alternaria solani through the use of photodynamic inhibition. In particular, magnesium chlorophyllin and disodium EDTA were used and their combination was shown to arrest the growth of Alternaria solani.

[0116] In the experiments, A. solani mycelia were grown in liquid medium for 24 hours. Pellets of mycelia (mean diameter 2 mm) were incubated with 100 μM magnesium chlorophyllin and 0 to 5 mM disodium EDTA for 100 minutes. The sample uses 395nm (the energy flow is 106.6J cm -2 ) was irradiated for 120 minutes, and the radial growth of mycelial plaques on the agar medium was measured after 7 days. Samples were considered dead if no growth was observed after 7 days on the agar plates. The table below summarizes the results:

[0117] Table 2: Results for the percentage of dead A. solani mycelium

[0118] deal with die% ...

Embodiment 3

[0121]In this example, the application of a porphyrin photosensitizer compound and a chelating agent inhibits the growth of the Botrytis cinerea fungus that causes Gray Mold by using photodynamic inhibition. In particular, magnesium chlorophyllin and disodium EDTA were used and their combination was shown to arrest the growth of Botrytis cinerea.

[0122] In the experiments, B. cinerea mycelia were grown in liquid medium for 48 hours. Pellets of mycelia (mean diameter 2 mm) were incubated with magnesium chlorophyllin (with or without 5 mM disodium EDTA) for 100 minutes. The sample uses 395nm (the energy flow is 106.6Jcm -2 ) was irradiated for 120 minutes, and the radial growth of mycelial plaques on the agar medium was measured after 7 days. Samples were considered dead if no growth was observed after 7 days on the agar plates. The table below summarizes the results:

[0123] Table 3: Results for the percentage of dead B. cinerea mycelium

[0124] deal with di...

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Abstract

There is provided a method for inhibiting growth of a microbial pathogen of a plant. The method comprises the following steps: applying to the plant a combination including a nitrogen-bearing macrocyclic compound which is a singlet oxygen photosensitizer selected from the group consisting of a porphyrin, a reduced porphyrin and a mixture thereof; and a chelating agent to increase permeability of the microbial pathogen to the nitrogen-bearing macrocyclic compound; and exposing the plant to light to activate the nitrogen-bearing macrocyclic compound and generate reactive singlet oxygen species.

Description

technical field [0001] The technical field generally relates to the photodynamic inhibition of microbial pathogens in plants using mixtures and compositions comprising photosensitizer compounds. More particularly, the technical field relates to nitrogen-containing macrocyclic compounds and compositions thereof for the photodynamic inhibition of microbial pathogens, such as fungal or bacterial pathogens, in plants. The nitrogen-containing macrocyclic compound may be a porphyrin compound or a reduced porphyrin compound. Background technique [0002] Photodynamic inhibition of microbial pathogens involves exposing photosensitizers to light to generate reactive oxygen species (ROS), such as singlet oxygen, which can have deleterious effects on microbial pathogens. Existing photodynamic suppression techniques and applications suffer from various drawbacks. Contents of the invention [0003] Described herein are various compositions and methods for the photodynamic suppression...

Claims

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Application Information

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IPC IPC(8): A01N43/90A01N31/02A01N37/44A01P3/00C07C229/16C07D487/22
CPCA01N37/44A01N43/16A01N43/64A01N59/06A01N59/16A01N59/20A01G7/045A01G7/06A01N37/46A01N43/90A01N55/02
Inventor M·费费尔K·普莱策J·刘B·纳什K·K-S·吴Y·特拉佐诺M·J·格吕克
Owner SUNCOR ENERGY INC
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