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Application of ccdc25 in predicting breast cancer prognosis and inhibiting distant organ metastasis of breast cancer

A breast cancer and prognosis technology, applied in the field of biomedicine, can solve the problems of lack of tumor metastasis mechanism and treatment methods, insufficient research on the biological behavior of malignant tumors, etc., and achieve the effect of inhibiting metastasis, simple operation and convenient detection.

Active Publication Date: 2022-02-25
崧铂(广州)生物医药有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even with a combination of multiple treatment methods, recurrence and metastasis are still extremely difficult problems. The reason is that we have insufficient research on the biological behavior of malignant tumors in the past, and there is even a lack of treatment methods that directly target the mechanism of tumor metastasis.

Method used

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  • Application of ccdc25 in predicting breast cancer prognosis and inhibiting distant organ metastasis of breast cancer
  • Application of ccdc25 in predicting breast cancer prognosis and inhibiting distant organ metastasis of breast cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Correlation between the expression of CCDC25 in tumor cells and the prognosis of breast cancer patients

[0021] Collect the primary tumor tissues of breast cancer patients in the hospital, and make tissue sections through fixation, dehydration, embedding, sectioning, etc.; deparaffinize, hydrate, antigen repair, and seal the pathological sections, and pass CCDC25 monoclonal antibody and tissue sections Incubation was carried out; the tissue sections were detected by DAB chromogenic method; and the expression changes of tumor cells CCDC25 were recorded. The result is as figure 1 As shown, by analyzing the expression of CCDC25 in breast cancer tissue and the survival of breast cancer liver metastasis, it was found that the expression level of CCDC25 was significantly related to the prognosis: the lower the expression of CCDC25, the higher the survival rate of patients with liver metastasis of breast cancer; the higher the expression of CCDC25, the higher the s...

Embodiment 2

[0022] Example 2: Tumor cell knockdown of CCDC25 can significantly inhibit tumor liver metastasis.

[0023] Construction of tumor cells knocking down CCDC25 expression: Infect breast cancer MDA-MB-231 cell lines with shRNA virus containing CCDC25 and negative control shluc virus respectively. After 48 hours of infection, select with 2.5ug / ml puromycin for 2 weeks to obtain stability cell line. The normal MDA-MB-231 cell line and the CCDC25 knocked down MDA-MB-231 cell line were mixed with 1×10 6 The amount of / 50ul was injected into the spleen of mice. After 30 days, the mice were placed under PET-CT to observe the liver metastasis of tumor cells. The result is as figure 2 As shown, the blank control group (UT) and the negative control group (shluc) can obviously have liver metastasis lesions in the liver of the mice, indicating that liver metastasis has occurred; while the experimental group (sh CCDC25 1 、sh CCDC25 2 ) No liver metastases were found. This suggests that ...

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Abstract

The invention discloses the application of the membrane protein CCDC25 in predicting the prognosis of breast cancer or inhibiting the distant organ metastasis of breast cancer and a kit, the kit includes reagents for detecting the expression of the membrane protein CCDC25 in breast cancer patients; in addition, the membrane protein CCDC25 neutralizing antibody can inhibit the metastasis of breast cancer cells. The kit of the invention assists the existing prognosis diagnosis to improve the survival rate of patients; the invention also provides a new treatment scheme for breast cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and particularly relates to the application of CCDC25 in predicting the prognosis of breast cancer and inhibiting distant organ metastasis of breast cancer. Background technique [0002] According to CA2018 research data, among the estimated 18.1 million new malignant tumor patients in 2018, 2.09 million are breast cancer patients, accounting for about 1 / 4 of the total number of new female malignant tumors. Leading to the highest number of deaths among malignant tumors. Among the deaths of breast cancer patients, distant metastasis of tumors is the main cause of death from breast cancer. Therefore, predicting the prognosis of breast cancer patients and inhibiting distant metastasis of tumors as early as possible is the key to improving the survival and efficacy of patients. [0003] The current treatment for patients with advanced breast cancer mainly includes radiotherapy, chemotherapy, en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574A61K39/395A61P35/04
CPCG01N33/57415G01N33/57484A61K39/39533A61P35/04G01N2800/52
Inventor 宋尔卫苏士成杨林槟陈嘉宁张晓倩李嘉倩李和亮
Owner 崧铂(广州)生物医药有限责任公司
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