A strain of Sphingosine polyphaga and its application

A technology of sphingosine bacteria and microbial strains, applied in the field of microorganisms, can solve the problem of not effectively degrading cellulose and the like, achieve high economic and social benefits, increase the degradation rate, and improve the effects of soil microecology

Active Publication Date: 2022-06-03
ANHUI WANNAN TOBACCO LEAF +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For this reason, the embodiment of the present invention provides a strain of Sphingobacterium multivorum and its application to solve the problem that there is no effective cellulose-degrading strain in the prior art

Method used

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  • A strain of Sphingosine polyphaga and its application
  • A strain of Sphingosine polyphaga and its application
  • A strain of Sphingosine polyphaga and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1. Isolation and identification of cellulolytic bacteria

[0037] 1. Isolation of Cellulolytic Bacteria

[0038] The Sphingobacterium multivorum of the embodiment of the present invention was isolated from the soil of Xuancheng, Anhui. The method for the separation of Sphingobacterium multivorum adopts the conventional method in the art for screening new strains, the soil circulation separation combined with the dilution plate purification method. Specific steps are as follows:

[0039]200g of soil collected from Xuancheng, Anhui and 10g of straw were weighed and put into the circulation enrichment device. 1500mL of inorganic salt liquid culture medium after sterilization at 121°C for 15min was pumped into the fluidized bed with a peristaltic pump, the speed of the peristaltic pump was 170rpm, and the air pump exhaust volume was 2L·min -1 , replenish the inorganic salt medium solution into the fluidized bed at regular intervals, and periodically add the circu...

Embodiment 2

[0052] Embodiment 2, Sphingosine polyphaga produces cellulase, hemicellulase activity

[0053] 1. Preparation of crude enzyme solution

[0054] Sphingomyelinum polyphaga was inoculated into straw cellulase-producing medium for cultivation. After culturing for 6 days, 7 mL of the liquid was taken out into a centrifuge tube, centrifuged at 4°C and 5000 rpm for 10 min, and the supernatant was taken as the crude enzyme liquid.

[0055] 2. Determination of cellulase activity

[0056] Take 4 clean and dried test tubes, numbered 1-4, add 1.5 mL of 0.5% sodium carboxymethyl cellulose solution to each test tube, add 2 mL of DNS solution to No. 1 test tube, and put the 4 test tubes into Preheat in a constant temperature water bath at 50 °C for 10 min; add 0.5 mL of the above crude enzyme solution to each test tube, keep the constant temperature water bath at 50 °C, and the reaction time is 30 min. Immediately after the reaction was terminated, 2 mL of DNS solution was added to the ot...

Embodiment 3

[0059] Example 3. Manganese peroxidase, laccase and lignin catalase activities of Sphingosine polyphaga

[0060] 1. Preparation of crude enzyme solution

[0061] Take 5 g of 1-2 cm straw segments, add 50 mL of straw ligninase-producing medium to them, and sterilize at 121° C. for 15 min. The Sphingosine polyphaga was inserted into the lignin enzyme production medium, placed in a 30°C incubator for cultivation, and water was regularly replenished to maintain the initial humidity. On the 6th day, take 1g of straw and add 20ml of 0.2mol·L -1 Acetic acid buffer solution (pH=5.0) was placed on a constant temperature oscillator at 30° C. for leaching for 2 hours, and the obtained leaching solution was the crude enzyme solution.

[0062] 2. Determination of manganese peroxidase, laccase and lignin catalase activities

[0063] To 3.4mL 0.11mol L -1 Lactic acid-sodium lactate buffer solution (pH=4.5) was added with 0.1mL40mmol·L -1 MnSO 4 The solution was preheated in a constant ...

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Abstract

The embodiment of the invention discloses the application of cellulolytic bacteria of Sphingobacterium multivorum in degrading cellulose. The embodiment of the present invention also discloses a cellulose degrading enzyme preparation, which contains the fermentation broth of Sphingobacterium multivorum. The Sphingobacterium multivorans in the embodiment of the present invention has the function of efficiently degrading the crop straws in the soil and water, so that the crop straws in the soil and water can be degraded quickly and efficiently.

Description

technical field [0001] The embodiments of the present invention relate to the technical field of microorganisms, in particular to a Sphingosine polyphaga and applications thereof. Background technique [0002] Agricultural products and food safety issues are the primary issues related to the national economy and people's livelihood. With the consumption of resources, people are facing many problems such as shortage of land resources, excessive use of chemical fertilizers and pesticides, serious heavy metal and organic pollution, and improper disposal of straw waste. It poses a great safety hazard to agricultural products and food, and may lead to more serious environmental pollution. Crop straw, as a natural associated product of agricultural products, will release a large amount of biological organic matter, as well as nutrients such as nitrogen, phosphorus and potassium after it is decomposed. Returning straw in-situ to the field is one of the important means of comprehen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08C12R1/01
CPCC12N1/20C05F11/08C12R2001/01C12N1/205Y02W30/40
Inventor 董祥洲胡丽娜朱启法
Owner ANHUI WANNAN TOBACCO LEAF
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