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Near-infrared II tumor primary lymph node operation navigation system and building method thereof

A surgical navigation and near-infrared technology, applied in the field of biological fluorescence imaging, can solve the problems of low luminous efficiency of probes, limited adjustable band, poor stability, etc., and achieve the effects of high luminous efficiency, improved accuracy and good stability

Active Publication Date: 2020-05-19
JILIN UNIV FIRST HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, solve the shortcomings of current probes such as low luminous efficiency, poor stability, and limited adjustable band; on the other hand, develop supporting imaging equipment to realize the surgical navigation system in the second near-infrared region

Method used

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  • Near-infrared II tumor primary lymph node operation navigation system and building method thereof
  • Near-infrared II tumor primary lymph node operation navigation system and building method thereof
  • Near-infrared II tumor primary lymph node operation navigation system and building method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0082] This example introduces the imaging of the primary lymph node of the mouse tumor and the navigation resection operation using the complex of albumin fragment III and cyanine dye. Firstly, tumor mice with lymph node metastasis were established by luciferase transgenic tumor cells, and then the metastatic primary lymph nodes were imaged using albumin fragment III and cyanine dye complex probe (intratumoral injection of the probe, and Waiting for it to slowly light up the first-level lymph nodes), you can see that the second-level near-infrared imaging can clearly distinguish the first-level lymph nodes and perform precise surgical navigation; after the operation, the resected first-level lymph nodes with tumor metastasis have been determined by bioluminescence. as attached Figure 12 (Refer to Substantive Examination References Figure 12 ) shown.

[0083] Further, the simple cyanine molecules currently used in clinical practice were selected for the near-infrared secon...

Embodiment 2

[0085] Based on the NIR-IIb quantum dot probe and the near-infrared second region surgical navigation system, the first-level lymph node resection of U87 subcutaneous tumor is performed with navigation, as shown in the attached Figure 14 (Refer to Substantive Examination References Figure 14 ), attached Figure 14 (Refer to Substantive Examination References Figure 14 ), a is the lymph node imaging image of U87 subcutaneous tumor model mice undergoing first-level lymph node navigation resection, and b is the relationship diagram of time signal enrichment;

[0086] From attached Figure 14 (Refer to Substantive Examination References Figure 14 ) It can be seen that when injecting NIR-IIb quantum dot probes into the tumor site, we found that the probes can quickly reach the first-level lymph nodes, and the signal can reach the peak in 4 hours, and the enrichment in 2 to 10 minutes The signal is sufficient for navigating surgical resection.

[0087] Further, imaging and ...

Embodiment 3

[0090] as attached Figure 16 (Refer to Substantive Examination References Figure 16 ), a mouse subcutaneous tumor model was established using luciferase transgenic 4T1 tumor cells, attached Figure 16 (Refer to Substantive Examination References Figure 16 ), a is the tumor model planted with luciferase-transfected 4T1 cells, b is the imaging of donor-receptor-donor molecules and NIR-IIb quantum dots on the primary lymph nodes of the tumor, and c is the effect of lymph node resection by navigation surgery picture.

[0091] From attached Figure 16 (Refer to Substantive Examination References Figure 16 ) can be seen, through the detection of fluorescein, it can be confirmed that the tumor has not metastasized to lymph nodes. In the absence of lymph node metastasis, we inject quantum dots into the tumor, and we can clearly distinguish the first-level lymph nodes next to the tumor, and perform navigation surgery for clear first-level lymph node resection of the tumor.

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Abstract

The invention discloses a near-infrared II tumor primary lymph node operation navigation system and a building method thereof. The near-infrared II tumor primary lymph node operation navigation systemis built by a detection camera, prisms, a filter lens, an operation platform and a laser generator, and also comprises a compound probe used for detection of the detection camera, wherein the compound probe is formed by carrying out compounding and wrapping by adopting cyanine dye and albumin fragments, or is formed by carrying out wrapping by adopting a polyacrylic acid copolymer based on quantum dots with emission wavelengths of 1500 nanometers or more. According to the whole navigation system, a complete set of equipment is selected, and the operation navigation purpose of wide-field imaging of primary lymph nodes of small animal tumors can be achieved; and the fluorescent probe is high in luminous efficiency, good in stability, good in biocompatibility, the wave band is adjustable ininfrared II, and thus the operation navigation system has a clinical transformation value.

Description

technical field [0001] The invention relates to bioluminescence imaging technology, in particular to a near-infrared second zone tumor primary lymph node surgical navigation system and a construction method thereof. Background technique [0002] At present, the development of biological imaging technology gives researchers more abundant means to understand the life process and develop effective medical methods. Among many imaging methods, fluorescence imaging is the most widely used imaging technology among all imaging technologies, which helps researchers use appropriate fluorescent probes to perform targeted imaging of cells, tissues, etc. Traditional fluorescence imaging mainly focuses on visible light (400-700 nm) and near-infrared (700-1000 nm) bands. Due to the influence of tissue autofluorescence and scattering, this band cannot achieve a good penetration depth and Imaging contrast. Therefore, in recent years, people have developed fluorescence imaging technology in...

Claims

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Application Information

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IPC IPC(8): A61B34/20
CPCA61B34/20A61B2034/2055
Inventor 朱守俊陈小元田蕊张明曦梁永晔
Owner JILIN UNIV FIRST HOSPITAL
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