Primer probe composition, kit and detection method for detecting methylation of human ADD3 and CDH23 genes

Abstract

The invention provides a primer probe composition, a kit and a detection method for detecting methylation of human ADD3 and CDH23 genes. The primer probe composition comprises a first specific primerand a first specific probe for detecting methylation of the human ADD3 gene, a second specific primer and a second specific probe for detecting methylation of the human CDH23 gene, and a third specific primer and a third specific probe for detecting a reference gene beta-actin. According to the primer probe composition, the kit and the detection method disclosed by the invention, specific detection of the methylation of the human ADD3 and CDH23 genes can be carried out simultaneously, accuracy of early screening and diagnosis of human prostate cancers can be realized, and the primer probe composition, the kit and the detection method are technologically advantaged in high sensitivity, high specificity, low cost, high throughput, simple and convenient operation, short experimental period and lower detection cost of genes.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer probe composition, a kit and a detection method for detecting the methylation of human ADD3 and CDH23 genes. Background technique [0002] Prostate cancer is a malignant tumor with high incidence in men. In the United States, the incidence of prostate cancer ranks first among male malignant tumors, and its mortality rate is second only to lung cancer. With changes in life, eating habits, and the environment, the incidence of prostate cancer in my country has increased rapidly in recent years. In developed cities such as Shanghai and Beijing, prostate cancer has become the number one malignant tumor of the male urinary system. [0003] At present, the early detection methods of prostate cancer are mainly the detection of the concentration of prostate cancer antigen PSA in serum and digital rectal examination. Patients with abnormal tests require further prostate ...

AI Technical Summary

Problems solved by technology

This existing technology relies on the exchange chromatographic column to achieve peak separation of methylated DNA detection signal, which is tantamount to increasing the detection cost of methylated DNA detection

Moreover, the technical solution disclosed in this prior art is not suitable for rapid screening and diagnosis of whether a patient suffers from prostate cancer in a hospital

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