Device and method for dyeing retinal vessels of lead-exposed mouse

A technology of mouse retina and retina, which is applied in the field of experimental devices, can solve problems such as the evaluation of unleaded blood vessel damage, and achieve the effects of simple processing, simple structure, and reduced damage

Pending Publication Date: 2020-05-12
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This model of vascular development has been mostly used in the research on the effects of hypoxia and diabetes on angiogenesis, but few researchers have used it to assess vascular damage of environmental pollutant lead

Method used

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  • Device and method for dyeing retinal vessels of lead-exposed mouse
  • Device and method for dyeing retinal vessels of lead-exposed mouse
  • Device and method for dyeing retinal vessels of lead-exposed mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0086] Such as Figure 1-Figure 6 As shown, the device for dyeing retinal blood vessels in lead-exposed mice of this embodiment includes:

[0087] A box body 1, the upper end of the box body 1 is open, and a hemispherical groove 4 is fixed inside the box body 1;

[0088] A box cover 2, the box cover 2 is arranged on the upper end of the box body 1 in order to close the upper end opening of the box body 1;

[0089]And the middle layer 3, the middle layer 3 is parallel to the bottom surface of the box body 1 and detachably arranged in the inside of the box body 1, the middle layer 3 is provided with a shape that penetrates up and down and matches the size of the retina. The first through hole 5 of the cylindrical structure is provided with a plurality of radially extending chute 6 at intervals along its circumference on the outer edge of the first through hole 5 , and each of the chute 6 is provided with The slide rail 7 and the slide bar 8 that is slidably connected on the sl...

experiment example

[0117]Set control group mice (do not carry out lead poisoning), lead poisoning group mice (lead dose is 0.7mg / kg mice) and lead poisoning group mice (lead dose is 7mg / kg mice), adopt respectively The staining method of embodiment 1, carries out mouse retinal blood vessel staining and mouse retinal blood vessel analysis, specifically as Figure 4-Figure 11 shown.

[0118] Depend on Figure 7-Figure 14 It can be seen that after the newborn mice in the control group and PbAC lead exposure group were exposed to PbAC orally, the blood vessel density and branch points increased with the increase of lead exposure concentration, and the changes in blood vessel density and total branch points had statistical significance. Differences between exposure groups. Compared with the control group WT, * P** P<0.01 (n=5 / genotype / group).

[0119] In summary, the present invention uses the above-mentioned device for dyeing retinal blood vessels of lead-exposed mice to perform staining, and pr...

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Abstract

The invention discloses a device and a method for dyeing retinal vessels of a lead-exposed mouse, which belong to the technical field of experimental devices. The dyeing device comprises a box body, abox cover and a middle layer, wherein the middle layer is provided with a first through hole which is through up and down, matched with the retina in size and of a cylindrical structure; a pluralityof sliding grooves extending in the radial direction are formed in the outer edge of the first through hole at intervals in the circumferential direction of the first through hole; a sliding rail anda sliding rod are arranged in each sliding groove; two bearing belts are detachably connected between each sliding rod and the inner wall face, on the opposite side, of the first through hole; and themultiple bearing belts intersect with one another to form a containing cavity used for containing the retina. The invention also discloses a dyeing method by using the device for dyeing the retinal vessels of the lead-exposed azithromycin. The dyeing device is simple in structure, the retina does not need to be repeatedly moved or flattened in the fixing, permeating, dyeing and photographing processes of the mouse retina, and damage to the retina is reduced.

Description

technical field [0001] The invention relates to a device and a method for dyeing retinal blood vessels of lead-exposed mice, belonging to the technical field of experimental devices. Background technique [0002] The mortality rate of cardiovascular diseases in China ranks first in the composition of disease deaths. Injury during vascular development is an early risk factor for cardiovascular disease in adulthood, and abnormalities in early angiogenesis have cardiovascular effects throughout the life course. The most important content in the study of vascular development is the change of angiogenesis and remodeling. The angiogenesis process is regulated by a variety of angiogenesis factors such as VEGF, Ang2, bFGF and IGF-1, which bind to endothelial cell receptors, initiate intracellular signaling systems, and activate endothelial cells. In this process, it has been found that NO is also an important part of VEGFA regulating angiogenesis. Epidemiological studies have sho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/31G01N21/64
CPCG01N1/31G01N21/6458G01N21/6486G01N2001/302G01N2001/305
Inventor 孙易何云梁丹刘耘耿芳李慧瑶
Owner GUILIN MEDICAL UNIVERSITY
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