Total iron detection kit and preparation method thereof

Abstract

The invention discloses a total iron detection kit. The total iron detection kit comprises a reagent R1 and a reagent R2. The reagent R1 is prepared from the following components: a Tris buffer solution, NaCl, trehalose, triton, hydroxylamine hydrochloride, sodium azide and Arabic gum. The reagent R2 comprises the following components: a Tris buffer solution, a latex-coated ferrous azine solutionand ascorbic acid. The preparation method of the total iron detection kit comprises the following steps: A) preparing the reagent R1: adding purified water into a blending tank A, sequentially addingthe components of the reagent R1 into the blending tank, mixing and stirring, regulating the pH value of the solution, and filtering by using a microporous filter membrane to obtain the reagent R1; and B) preparing the reagent R2: adding purified water into a blending tank B, sequentially adding the components of the reagent R2 into the blending tank B, mixing and stirring, adjusting the pH valueof the solution, and filtering by using a microfiltration membrane to obtain the reagent R2. By adopting the scheme disclosed by the invention, the detection efficiency is improved, and the clinical disease detection cost is reduced.

Description

technical field [0001] The invention relates to a preparation method of a total iron detection kit, in particular to a total iron detection kit and a preparation method thereof. Background technique [0002] In blood testing, the detection of total iron binding capacity is a routine item of blood testing, and some clinical diseases can be judged by detecting the strength of total iron binding capacity in blood. [0003] Specifically, existing clinical studies have shown that diseases such as iron deficiency anemia, liver cirrhosis, and uremia are likely to be important factors that cause a significant increase in total iron binding capacity in the blood. [0004] Therefore, detecting the strength of total iron binding capacity in blood has important clinical diagnostic significance for the prevention and diagnosis of some clinical diseases. [0005] However, at present, the main way to detect total iron binding capacity clinically is still using atomic absorption method, an...

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Problems solved by technology

[0006] The above-mentioned detection methods are not only backward, but also have a long detection period, and the detection cost is relatively high, such as atomic absorption method, which not only increases the cost of clinical detection but also reduces the detection efficiency

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