Production method for high-content galactooligosaccharide

The technology of a galacto-oligosaccharide and a production method is applied in the production field of high-content galacto-oligosaccharide, and can solve the problems of low purity of galacto-oligosaccharide, decreased lactose content, low synthesis efficiency and the like

Inactive Publication Date: 2020-05-08
安徽民祯生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a production method of high-content galacto-oligosaccharides, which solves the problem of low purity and low synthesis efficiency of galacto-oligosaccharides prepared by enzymatic synthesis technology The conversion rate of galacto-oligosaccharides is suppressed, so that the content of lactose will continue to decline

Method used

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  • Production method for high-content galactooligosaccharide
  • Production method for high-content galactooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] S1. Preparation of enzyme fermentation broth: use 300g / L lactose solution as raw material, add β-galactosidase in the lactose solution, add 10U of β-galactosidase per gram of lactose raw material, put it in a high-speed mixer, Stir evenly at a temperature of 25° C., with a pH value of 6. After cultivating for 30 hours, when the enzyme reacts until the mass percentage of glucose in the total sugar is 10%, the reaction work is terminated, and finally the enzyme fermentation broth is obtained, waiting for use;

[0021] S2. Preparation of galactooligosaccharide serum: put the enzyme fermentation broth obtained in S1 into a high-speed mixer, and then add a galactosidase solution with a concentration of 200g / L. After stirring and reacting for 15 hours, the obtained solution was centrifuged to remove the internal bacteria, and finally the galacto-oligosaccharide serum was obtained, which was left to stand for later use;

[0022] S3. Preparation of galacto-oligosaccharide syrup...

Embodiment 2

[0025] S1. Preparation of enzyme fermentation broth: use 350g / L lactose solution as raw material, add β-galactosidase in the lactose solution, add 20U of β-galactosidase per gram of lactose raw material, put it in a high-speed mixer, Stir evenly at a temperature of 30° C., with a pH value of 6.5. After culturing for 35 hours, when the enzyme reacts until the mass percentage of glucose in the total sugar is 12%, the reaction work is terminated, and finally the enzyme fermentation broth is obtained, waiting for use;

[0026] S2. Preparation of galacto-oligosaccharide serum: put the enzyme fermentation liquid obtained in S1 into a high-speed mixer, and then add a galactosidase solution with a concentration of 400g / L. After stirring and reacting for 18 hours, the obtained solution was centrifuged to remove the internal bacteria, and finally the galacto-oligosaccharide serum was obtained, which was left to stand for later use;

[0027] S3. Preparation of galacto-oligosaccharide syr...

Embodiment 3

[0030] S1. Preparation of enzyme fermentation broth: use 400g / L lactose solution as raw material, add β-galactosidase in the lactose solution, add 30U of β-galactosidase per gram of lactose raw material, put it in a high-speed mixer, Stir evenly at a temperature of 35° C., with a pH value of 7. After 40 hours of cultivation, when the enzyme reacts until the mass percentage of glucose in the total sugar is 15%, the reaction work is terminated, and finally the enzyme fermentation broth is obtained, waiting for use;

[0031] S2. Preparation of galactooligosaccharide serum: put the enzyme fermentation broth obtained in S1 into a high-speed mixer, and then add a galactosidase solution with a concentration of 500g / L. After stirring and reacting for 20 hours, the obtained solution was centrifuged to remove the internal bacteria, and finally the galacto-oligosaccharide serum was obtained, which was left to stand for later use;

[0032] S3. Preparation of galacto-oligosaccharide syrup:...

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Abstract

The invention discloses a production method for high-content galactooligosaccharide. The production method comprises the following steps: S1, preparation of an enzyme fermentation liquor: with a lactose solution with a concentration of 300-400 g/L as a raw material, adding beta-galactosidase into the lactose solution, wherein 10-30 U of the beta-galactosidase is added into each gram of a lactose raw material, putting an obtained mixed solution into a high-speed stirrer, carrying out uniform stirring at 25-35 DEG C, wherein a pH value is 6-7, then carrying out culturing for 30-40 h, and when the mass percentage of glucose accounts for 10-15% of the total amount of sugar through an enzyme reaction, stopping reaction work so as to finally obtain an enzyme fermentation liquor for subsequent usage. The invention relates to the technical field of production of the galactooligosaccharide. The production method for high-content galactooligosaccharide has the following advantages: beer yeast isadded in the preparation process of the galactooligosaccharide, so the conversion rate of the galactooligosaccharide can be increased, and short reaction time and high synthesis rate are achieved; byusing of an ultrafiltration membrane and a nanofiltration membrane, the purity of the galactooligosaccharide can be greatly improved; meanwhile, the production cost is reduced to a certain extent, and the actual production requirements are met.

Description

technical field [0001] The invention relates to the technical field of galacto-oligosaccharide production, in particular to a production method of high-content galacto-oligosaccharide. Background technique [0002] Galacto-oligosaccharides are functional oligosaccharides with natural properties, and their molecular structure is generally 1-7 galactosyl groups connected to galactose or glucose molecules, that is, Gal-(Gal)n-Glc / Gal( n is 0-6), in nature, there is a trace amount of GOS in the milk of animals, while the content in human breast milk is more, and the establishment of the bifidobacteria flora in the baby's body largely depends on the GOS components in breast milk. In nature, there is a small amount of galacto-oligosaccharides in animal milk, and the content of galacto-oligosaccharides is slightly more in breast milk. The sweetness of galacto-oligosaccharides is relatively pure, the calorific value is low (7.1J / g), and the sweetness is 20%-40% of that of sucrose. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/12C12P19/00C07H3/04C07H3/06C07H1/06C08B37/00C12R1/865
CPCC07H1/06C07H3/04C07H3/06C08B37/0003C08B37/0036C12P19/00C12P19/12C12P19/14
Inventor 宋建民王德海宛荣生张琴王颂黄祥君
Owner 安徽民祯生物工程有限公司
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