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A method for producing seedlings of Botrytis elongatum by artificial sexual breeding

A long-stem grape fern algae and seedling technology, which is applied in the fields of botany equipment and methods, applications, seaweed cultivation, etc., can solve the problems of artificial sexual reproduction of sea grapes that have not been reported, and achieve high reproductive efficiency without being affected by seasons. The effect of restriction

Active Publication Date: 2021-11-09
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sea grapes can reproduce sexually under certain natural conditions, but artificial sexual reproduction of sea grapes has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Selection of algae species: select sea botrytis algae that have grown for more than 2 months and are growing well, use a fine brush to remove algae and attachments on the surface of the algae, and then rinse repeatedly with sterilized filtered seawater 4-6 times. The algae were placed under the conditions of 28°C, light intensity 3000Lx, and photoperiod 12D:12L.

[0038] Induction: Place the sea grape algae at 18°C ​​for 3 days, then place the algae in sterilized seawater at 28°C, light intensity 4000Lx, photoperiod 12D:12L, every 7 days Change the sea water once.

[0039] Observation of sexual reproduction characteristics: every 7 days, take sea grape algae and observe the changes on the surface of the algae with a stereo microscope until the surface of the algae has a network structure and the base of the globular branchlet grows a germination tube, indicating that the sea grape is sexually mature .

[0040] Adhesive base selection: choose about 3 mm of gravel to la...

Embodiment 2

[0046]Selection of algae species: select sea grape algae that have grown for more than 3 months and are growing well, use a fine brush to remove algae and attachments on the surface of the algae, and then rinse repeatedly with sterilized filtered seawater 4-6 times. The algae were placed under the conditions of 28°C, light intensity 3000Lx, and photoperiod 12D:12L.

[0047] Induction: Place the sea grape algae at 15°C for 1 day, then place the algae in sterilized seawater at 28°C, light intensity 4000Lx, photoperiod 12D:12L, and cultivate it every 7 days Change the sea water once.

[0048] Observation of sexual reproduction characteristics: every 7 days, take sea grape algae and observe the changes on the surface of the algae with a stereo microscope until the surface of the algae has a network structure and the base of the globular branchlet grows a germination tube, indicating that the sea grape is sexually mature .

[0049] Attachment base selection: select cloth curtains...

Embodiment 3

[0055] Selection of algae species: select sea grape algae that have grown for more than 3 months and are growing well, use a fine brush to remove algae and attachments on the surface of the algae, and then rinse repeatedly with sterilized filtered seawater 4-6 times. The algae were placed under the conditions of 28°C, light intensity 3000Lx, and photoperiod 12D:12L.

[0056] Induction: place sea grape algae at 20°C for 6 days, then place the algae in sterilized seawater at 28°C, light intensity 4000Lx, photoperiod 12D:12L, and cultivate every 7 days Change the sea water once.

[0057] Observation of sexual reproduction characteristics: every 7 days, take sea grape algae and observe the changes on the surface of the algae with a stereo microscope until the surface of the algae has a network structure and the base of the globular branchlet grows a germination tube, indicating that the sea grape is sexually mature .

[0058] Adhesive base selection: choose about 2 mm of crushed...

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Abstract

This application belongs to the technical field of seaweed cultivation, and discloses a method for producing seedlings of Botrytis elongatum by artificial sexual breeding. Select the Botrytis elongatus seedlings that have grown for more than 2 months and have good growth, and clean the algae on the surface of the algae. , used as seed algae after pre-cultivation; seed algae are first induced by low temperature (15-20°C), then cultured under conventional conditions, and the water is changed regularly until sexual maturity is observed; the seed algae are moved into sterilized seedlings with attachment substrates In the pool, give a certain light stimulation, cultivate and release the male and female gametes, and then move the algae out of the nursery pool, and culture the fertilized eggs for 1-2 months to obtain artificial seedlings. The method of the present invention can utilize sexual breeding to produce Botrytis elongatum seedlings, is suitable for large-scale breeding of Botrytis elongatum seedlings, has high reproduction efficiency, and the seedling production is not limited by seasons.

Description

technical field [0001] The invention belongs to the technical field of seaweed cultivation, and in particular relates to a method for producing seedlings of Botrytis elongatum by artificial sexual breeding. Background technique [0002] Caulerpa lentillifera J. Agardh, also known as sea grape, belongs to the genus Caulerpa in the family Caulerpacae of Chlorophyta. Botrytis elongatus mainly grows on sandy land or reefs with slow currents in the tropical and subtropical intertidal sea areas of the Pacific Ocean. It is a warm-temperature large-scale economic green algae. Grape fern algae not only contains a large amount of amino acids, unsaturated fatty acids, minerals, and trace elements such as phosphorus, calcium, and magnesium, but also does not contain cholesterol, and its nutritional value is much higher than that of other seaweeds and vegetables. In addition, Botrytis elongatum also has a unique taste and is known as "green caviar". It is a potential health product with...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00
CPCA01G33/00Y02A40/80
Inventor 朱军鲍时翔邹潇潇孟天陈佐符青艳
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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