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Method for determining trace chlorophyll in high-organic-matter soil

A measurement method and chlorophyll technology, applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., to achieve good correlation and broaden the effect of research indicators

Inactive Publication Date: 2020-05-05
HUBEI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these applications require a relatively high content of chlorophyll and its derivatives

Method used

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  • Method for determining trace chlorophyll in high-organic-matter soil
  • Method for determining trace chlorophyll in high-organic-matter soil
  • Method for determining trace chlorophyll in high-organic-matter soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] S1. Material collection: select litter layer peat moss (0-10cm depth), shallow peat moss (10-20cm depth) and deep peat moss (20-25cm depth) and peat moss samples, vacuum freeze dryer at -80°C Freeze drying;

[0039] S2, extraction of chlorophyll: accurately weigh the peat sample to be tested after 0.2g freeze-drying, 0.025g peat moss sample in the container, then add 10ml of 95% (v / v) ethanol solvent in the container respectively, after sealing and sealing Extract chlorophyll at 25°C in the dark for 6 hours to prepare soil chlorophyll extract and sphagnum moss chlorophyll extract. Here, sphagnum chlorophyll extract is used as chlorophyll standard solution;

[0040] S3, UV-visible spectrophotometry to measure the content of chlorophyll: Utilize UV-visible spectrophotometry to measure the optical density of the sphagnum chlorophyll extract at wavelengths of 663nm and 645nm, and use the method of Lichtenthaler to calculate the chlorophyll in the sphagnum chlorophyll extrac...

Embodiment 2

[0052] S1, materials: choose the fallen osmanthus fragrans leaves ((0-10cm), be the dead osmanthus fragrans leaves that just fell and the slightly rotten osmanthus fragrans leaves), shallow osmanthus fragrans soil (10-20cm) and deep osmanthus fragrans soil (20- 25cm) and peat moss, freeze-dried at -80°C in a vacuum freeze dryer;

[0053] S2, the extraction of chlorophyll: accurately take by weighing the Osmanthus osmanthus leaf soil sample to be tested after 0.2g freeze-drying, 0.025g sphagnum moss sample is in the container, then respectively add 10ml of 95% (v / v) ethanol solvent in the container, seal After sealing, extract chlorophyll at 20°C in the dark for 6 hours to prepare osmanthus leaf soil chlorophyll extract and sphagnum moss chlorophyll extract. Here, sphagnum chlorophyll extract is used as chlorophyll standard solution (other plants can also be used as standards) ;

[0054] S3, the content of chlorophyll is measured by visible ultraviolet spectrum: with step S3 a...

Embodiment 3

[0060] S1. Materials: choose fallen bamboo leaves ((0-5cm), just fallen dead bamboo leaves and slightly rotten bamboo leaves), shallow bamboo leaf soil (5-10cm) and deep layers Bamboo leaf soil (10-15cm) and sphagnum moss, freeze-dried at -80°C in a vacuum freeze dryer;

[0061] S2, extraction of chlorophyll: Accurately weigh 0.2g freeze-dried bamboo leaf soil sample to be tested, 0.025g sphagnum moss sample in the container, then add 10ml of 95% (v / v) ethanol solvent in the container , after sealing and sealing, extract chlorophyll at 25° C. in the dark for 6 hours to prepare soil chlorophyll extracts of bamboo leaves to be tested and sphagnum chlorophyll extracts, where the sphagnum chlorophyll extracts are used as chlorophyll standard solutions;

[0062] S3, the content of chlorophyll is measured by visible ultraviolet spectrum: with step S3 among the embodiment 1;

[0063] S4, the three-dimensional fluorescence spectrum measurement of chlorophyll: with the step S4 in the ...

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Abstract

The invention discloses a method for determining trace chlorophyll in high-organic-matter soil. The method comprises the following steps: S1, taking materials: selecting a soil sample to be determinedand a green plant sample, and carrying out freeze drying; S2, extracting chlorophyll; S3, determining the optical density of the chlorophyll extracting solution of the green plant under the wavelength of 663 nm and 645 nm by utilizing an ultraviolet-visible spectrophotometry, and calculating the content of chlorophyll a in the chlorophyll extracting solution of the green plant by adopting a Lichtenthaler method; S4, measuring a three-dimensional fluorescence spectrum; S5, drawing a standard curve, and establishing a regression equation; and S6, measuring the content of chlorophyll in the soilto be determined. The low-background three-dimensional fluorescence spectrum is utilized, the problem that the absorption light of trace chlorophyll is greatly interfered by peat, high organic mattersoil and other colored backgrounds in the visible ultraviolet spectrum can be solved, the limitation that the determination of chlorophyll is only limited to green plants is broken through, and the method can be widely used for researching the process of green plant litter and humification.

Description

technical field [0001] The invention relates to the technical field of chlorophyll determination, in particular to a method for determining trace chlorophyll in soil with high organic matter. Background technique [0002] Chlorophyll is the main pigment for photosynthesis in green plants. The content determination of chlorophyll and its derivatives has been widely used in many fields such as studying photosynthesis, monitoring marine red tide and phytoplankton, studying the ripening mechanism of melons and fruits, and detecting chlorophyll derivative additives and color in food. But these applications require a relatively high content determination of chlorophyll and its derivatives. The determination of trace chlorophyll is rarely used in other fields except water research. Chlorophyll has a porphyrin structure with a special conjugated system. Porphyrin can be used as a molecular identification marker in some researches. Similarly, as long as there are green plants, chlo...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486G01N2021/6419
Inventor 卜贵军邸慧慧徐洁吴林宋益陈尧
Owner HUBEI UNIV FOR NATITIES
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