Composite micro-ecological preparation for purifying aquaculture water body and application of composite micro-ecological preparation

A composite micro-ecology and water purification technology, applied in microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problems of lack of strain synergistic performance and strain characteristics, and the degradation effect cannot achieve purification effect, so as to promote water The virtuous cycle of the ecosystem, the effect of improving the performance of the product to purify the water body, and the fast speed

Active Publication Date: 2020-05-05
MINJIANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the degradation effect of a single Bacillus in aquaculture cannot achieve a good purification effect. It can degrade ammonia nitrogen, nitrite and sulfide in water, but it cannot achieve a good purification effect. The active compound micro-ecological preparation can effectively reduce the COD and BOD in the water, reduce the concentration of ammonia nitrogen, nitrite nitrogen and sulfide in the water, thereby effectively improving the water quality
At present, the compound microecological products on the market are basically blindly mixed, lacking the synergistic performance and strain characteristics between strains

Method used

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  • Composite micro-ecological preparation for purifying aquaculture water body and application of composite micro-ecological preparation
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  • Composite micro-ecological preparation for purifying aquaculture water body and application of composite micro-ecological preparation

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preparation example Construction

[0031] In the present invention, the preparation method of the solid bacterial powder comprises the following steps: after activating the bacterial species, carry out continuous two-stage fermentation and concentration to obtain a concentrated bacterial liquid, and the Bacillus amyloliquefaciens JL-B05, Bacillus JL-B06, Bacillus subtilis LYN-1, and Bacillus licheniformis LYN-3 are mixed with light calcium carbonate and then spray-dried to obtain the solid bacteria powder.

[0032] In the present invention, the strains are inoculated in NB culture medium and cultivated for 15-25 hours to obtain a primary seed solution; the culture temperature is preferably 25-35°C, and the culture speed is preferably 150-350 r min -1 .

[0033] In the present invention, after the primary seed liquid is obtained, the primary seed liquid is transferred to the NB liquid medium and cultivated for 10-20 hours to obtain the secondary seed liquid. The inoculation amount of the first-stage seed liquid...

Embodiment 1

[0039] (1) Preparation of Bacillus amyloliquefaciens JL-B05 bacterial liquid and solid bacterial powder:

[0040] Activate the Bacillus amyloliquefaciens JL-B05 strain, pick a single colony, inoculate in 150ml NB medium (121°C, sterilize for 30min), 28°C, 180 r min -1 Under the condition of shaking culture for 20 h, the primary seed solution was obtained.

[0041] Preparation of secondary seed solution of Bacillus amyloliquefaciens JL-B05

[0042] Put the first-grade seed solution into a sterile seed tank, the inoculum amount is 0.5% ~ 1.0% (V / V); the culture conditions are: manually adjust the speed and air flow of ventilation to adjust DO (DO40%), and the culture temperature is 30℃~ 35℃, the speed is 200~350r·min -1 , tank pressure 0.05MPa; cultivation time 15~20 h. OD of secondary seed solution 600 The value is 0.8, at this time the strain activity is the strongest.

[0043] Preparation of Bacillus amyloliquefaciens JL-B05 fermentation broth

[0044] Put the secondar...

Embodiment 2

[0050] The preparation method of the Bacillus amyloliquefaciens JL-B05, Bacillus amyloliquefaciens JL-B06, Bacillus subtilis LYN-1, Bacillus licheniformis LYN-3 bacteria liquid and solid bacteria powder is the same as that in Example 1.

[0051] Mix the solid bacteria powder of the above-mentioned Bacillus amyloliquefaciens JL-B05, Bacillus amyloliquefaciens JL-B06, Bacillus subtilis LYN-1, and Bacillus licheniformis LYN-3 according to the mass ratio of 3:3:2:2 to form a solid Mix powder. The solid preparation of the growth-promoting compound microecological preparation is obtained after the carrier is mixed with the above-mentioned solid mixed bacterial powder at a mass ratio of 100:5, and the total viable bacteria concentration in the compound probiotic preparation is 2×10 9 cfu / g. The carrier is bran, coral sand and diatomaceous earth, and the mass ratio of the three carriers is 80:10:3.

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Abstract

The invention provides a composite micro-ecological preparation for purifying an aquaculture water body and an application of the composite micro-ecological preparation, and belongs to the field of microbial preparations. The preparation comprises bacillus amyloliquefaciens JL-B05, bacillus amyloliquefaciens JL-B06, bacillus subtilis LYN-1 and bacillus licheniformis LYN-3 in a mass ratio of (3-6):(3-8):(2-5):(2-6). The composite micro-ecological preparation for purifying an aquaculture water body has strong tolerance to harmful substances in the aquiculture water body, and has high degradationcapacity, high speed and remarkable water body purification effect. Meanwhile, the composite micro-ecological preparation can improve the survival rate and yield of aquiculture products, is a new-generation green environment-friendly product, and has a very important effect in protecting the aquiculture water body environment and promoting benign circulation of the water ecosystem.

Description

technical field [0001] The invention relates to a compound micro-ecological preparation for purifying aquaculture water and an application thereof, belonging to the field of microbial preparations. Background technique [0002] With the rapid development of the aquaculture industry and the continuous improvement of intensive management, harmful algae and pathogenic bacteria multiply in many aquaculture ponds, which seriously damages the aquaculture ecological environment and leads to frequent aquaculture diseases. In addition, the extensive use of antibiotics in aquaculture has also led to problems such as drug residues in animals and ecological imbalance in the water environment. Therefore, how to reduce the use of antibiotics and water pollution, and control the content of ammonia nitrogen and nitrite in water is an urgent problem to be solved in aquaculture production. [0003] As a new type of green and environment-friendly product, microecological preparations play an ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/10C12R1/07C02F103/20
CPCC02F3/341C02F2103/20C12N1/20
Inventor 袁宗胜刘芳李泳宁周丽丽陈建明
Owner MINJIANG UNIV
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