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Preparation method of palatable flavor enzymolysis fermentation wet feed for feed

A flavor enzyme, wet feed technology, applied in the field of feed, enzyme preparations and microorganisms

Inactive Publication Date: 2020-04-21
合肥五粮泰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the fermented feed products in China are relatively simple and biased towards microecology. On the one hand, the palatability of fermented feed and the digestion and pretreatment of feed still need to be improved.

Method used

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  • Preparation method of palatable flavor enzymolysis fermentation wet feed for feed
  • Preparation method of palatable flavor enzymolysis fermentation wet feed for feed
  • Preparation method of palatable flavor enzymolysis fermentation wet feed for feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] Step 1: Preparation of lactic acid bacteria seed solution

[0148] Add 3000g of glucose, 1000g of dry corn steep powder, 500g of beef extract, 1000g of yeast extract, 500g of sodium chloride, 200g of potassium dihydrogen phosphate, 60g of magnesium sulfate, and 25g of manganese sulfate into the seed tank. After being sterilized in minutes, cool to the culture temperature, insert the strains of lactic acid bacteria in shake flasks, and perform anaerobic culture for 24 hours to obtain 60 kg of Lactobacillus plantarum bacterial liquid, 10 kg of Lactococcus bacterial liquid, and 30 kg of Lactobacillus thermophilic bacterial liquid.

[0149] Step 2: Yeast seed liquid preparation:

[0150] Add 50g of yeast extract, 100g of peptone, 100g of glucose and 25g of sodium chloride to the seed tank, add water to make up to 5L, adjust the pH to 6.9-7.1, sterilize at 121°C for 30 minutes, cool to the culture temperature, and insert yeast Bacteria strains were cultured aerobically for ...

Embodiment 2

[0166] Step 1: Preparation of lactic acid bacteria seed solution

[0167] Add 4200g of glucose, 1400g of dry corn steep powder, 700g of beef extract, 1400g of yeast extract, 700g of sodium chloride, 280g of potassium dihydrogen phosphate, 84g of magnesium sulfate, and 35g of manganese sulfate into the seed tank. Cool to culture temperature after minute sterilization, insert lactic acid bacteria shake flask bacterial classification, anaerobic culture 24 hours, make Lactobacillus plantarum bacterial liquid 50kg, Lactococcus bacterial liquid 50kg, thermophilic Lactobacillus bacterial liquid 40kg.

[0168] Step 2: Yeast seed liquid preparation:

[0169] Add 50g of yeast extract, 100g of peptone, 100g of glucose and 25g of sodium chloride to the seed tank, add water to make up to 5L, adjust the pH to 6.9-7.1, sterilize at 121°C for 30 minutes, cool to the culture temperature, and insert yeast Bacteria strains were cultured aerobically for 24 hours to obtain 4 kg of baker's yeast see...

Embodiment 3

[0185] Step 1: Preparation of lactic acid bacteria seed solution

[0186] Add 1050g of glucose, 350g of dry corn steep powder, 175g of beef extract, 350g of yeast extract, 175g of sodium chloride, 70g of potassium dihydrogen phosphate, 21g of magnesium sulfate, and 8.75g of manganese sulfate into the seed tank. After sterilizing for 30 minutes, cool to the culture temperature, insert lactic acid bacteria strains in shake flasks, and perform anaerobic culture for 24 hours to obtain 20 kg of Lactobacillus plantarum and 15 kg of Lactobacillus thermophiles.

[0187] Step 2: Yeast seed liquid preparation:

[0188] Add 100g of yeast extract, 200g of peptone, 200g of glucose and 50g of sodium chloride to the seed tank, add water to make up to 10L, adjust the pH to 6.9-7.1, sterilize at 121°C for 30 minutes, cool to the culture temperature, and insert yeast Bacteria strains were cultured aerobically for 24 hours to obtain 2 kg of baker's yeast seed liquid and 8 kg of high-temperature...

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Abstract

The invention discloses a preparation method of palatable flavor enzymolysis fermentation wet feed for feed. The preparation method comprises the following steps: (1) respectively preparing a lactic acid bacteria strain solution, a yeast strain solution and a bacillus strain solution; (2) respectively preparing an L-aspartic acid fermentation broth and an L-phenylalanine fermentation broth; (3) preparing an aspartame synthetic liquid, wherein the L-aspartic acid fermentation broth prepared in the step (2), formic acid and magnesium oxide are sequentially added into a reaction kettle, stirringis started to completely dissolve the magnesium oxide, acetic anhydride is added, heating is performed to 50 DEG C, a constant temperature reaction is performed for 4 h, cooling is performed to 25 DEGC, the L-phenylalanine fermentation broth prepared in the step (2), methyl acetate and acetic acid are sequentially added, and stirring is performed for 7 h to obtain the aspartame synthetic liquid;and (4) preparing a solid-state acidified and glycolyzed feed, wherein substances prepared from clean water, a material A, a material B, a material C and a material D are mixed to directly produce a solid-state acidified and glycolyzed feed wet base, and fermentation enzymolysis is performed for a period of time to obtain the fermented wet material.

Description

technical field [0001] The invention belongs to the technical fields of feed, enzyme preparations and microorganisms, and relates to a method for preparing palatable flavor enzymolysis and fermentation wet feed for feeding. Background technique [0002] Micro-ecological fermented feed is a new type of feed, which can regulate the intestinal microbial community of livestock and poultry, improve the balance of digestive tract flora and enzymes in the animal body, and rapidly improve the body's disease resistance, metabolism, and digestion and absorption capabilities, thereby To achieve the dual role of prevention and treatment of digestive tract diseases and promote growth. Probiotics are pure biological products, without pollution and side effects to the environment. Therefore, the promotion and application of probiotics in feed additives is undoubtedly of great significance to the healthy growth of livestock and poultry, to ensuring the quality of livestock products and imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23L27/30A23K10/30A23K10/37A23K10/22A23K20/20A23K10/33A23K20/26A23K20/22A23K50/30A23K50/60A23K50/10C12N1/20C12N1/18C12P13/20C12P13/22C07K5/075C12R1/01C12R1/25C12R1/225C12R1/865C12R1/10C12R1/125C12R1/07
CPCA23K10/12A23K10/14A23L27/32A23K10/30A23K10/37A23K10/22A23K20/20A23K10/33A23K20/26A23K20/22A23K50/30A23K50/60A23K50/10C12N1/20C12N1/18C12P13/20C12P13/222C07K5/0613Y02P60/87
Inventor 程淦程勐万里孟龙薛芹
Owner 合肥五粮泰生物科技有限公司
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