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Leukocyte classification reagent

A white blood cell classification and reagent technology, applied in the direction of analysis materials, individual particle analysis, biological particle analysis, etc., can solve the problems of environmental pollution, complex reagent components, etc., and achieve the effect of simple formula

Inactive Publication Date: 2020-04-17
SONOSCAPE MEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reagent has complex components and contains dyes, which will pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1: The leukocyte classification reagent of the present invention and its application detection

[0030]

[0031] The physical and chemical properties of the reagent prepared by the above formula were tested, the pH was 7.0, the osmotic pressure was 396mOsm / kg, and the conductivity was 18ms / cm. Use the above reagents to test blood samples a (normal sample) and b (abnormal sample - immature granulocytes) on the SC-9000 automatic blood analyzer. The results are as follows figure 1 -a and figure 1 -b shown. Neu denotes neutrophils, Lym lymphocytes, Mon monocytes, Eos eosinophils and IG immature granulocytes. White blood cells are divided into neutrophils, lymphocytes, monocytes, eosinophils (and immature white blood cells) several types, each type of cell boundaries are clear.

[0032] Preparation does not use the formulated reagent (control) formula of polyol type nonionic surfactant of the present invention as follows:

[0033]

[0034]

[0035] Th...

Embodiment 2

[0036] Embodiment 2: The leukocyte classification reagent of the present invention and its application detection

[0037]

[0038] The reagent prepared according to the above formula, the physical and chemical properties of the test reagent, pH is 5.5, osmotic pressure is 525mOsm / kg, and conductivity is 22ms / cm. Using the above reagents to test blood samples c (high eosinophilic samples) and d (abnormal samples - immature granulocytes exist) on the SC-9000 automatic blood analyzer, the results are as follows figure 2 As shown in -c and 2-d, the white blood cells on the scatter diagram are divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils. In samples with immature granulocytes, immature granulocytes Also grouped individually.

[0039] Also prepare the reagent (control) according to Example 1, replace Tween-40 in the formula of this example with dodecyltrimethylammonium chloride, and use the reagent (control) to detect sample d, the ...

Embodiment 3

[0040] Embodiment 3: The leukocyte classification reagent of the present invention and its application detection

[0041]

[0042] Prepare the reagent according to the above formula, test the physical and chemical properties of the reagent, the pH is 8.0, the osmotic pressure is 685mOsm / kg, and the conductivity is 20ms / cm. Use the above reagents to test blood samples e (normal samples) and f (abnormal samples - there are immature granulocytes) on the SC-9000 automatic blood analyzer, the results are as follows image 3 As shown in -e and f, the white blood cells on the scatter diagram are divided into four clear cell groups of lymphocytes, monocytes, neutrophils and eosinophils. In the samples with immature granulocytes, the immature granulocytes are also separated In groups.

[0043] Also prepare the reagent (control) according to Example 1, replace Tween-60 in the formula of this example with dodecyltrimethylammonium chloride, and use the reagent (control) to detect samp...

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Abstract

The invention relates to the technical field of in vitro diagnosis, and discloses a leukocyte classification reagent. The leukocyte classification reagent comprises a polyoxyethylene type nonionic surfactant, a polyol type nonionic surfactant, a buffer solution, a conductivity supplement, an osmotic pressure regulator and a preservative. The reagent provided by the invention is simple in formula and dye-free, the main components only comprise two specific nonionic surfactants, and the defects of excessive cell damage or too small size and too large distribution span of the same kind of cells and failure in effective classification which are possibly caused by other reagents are avoided. According to the present invention, with the reagent, leukocytes can be divided into monocytes, lymphocytes, neutrophils (including basophils) and eosinophils with clear boundaries, and juvenile granulocytes can be classified.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a white blood cell classification reagent. Background technique [0002] Cells in human blood are roughly divided into three categories: red blood cells, white blood cells and platelets, among which white blood cells can be further divided into monocytes, lymphocytes and granulocytes (including neutrophils, eosinophils and basophils) five subcategories. Blood cells are produced in the bone marrow, differentiate from immature cells, mature, and then enter the peripheral blood to perform their respective functions. The proportions of various subtypes of white blood cells in the peripheral blood of normal healthy people are constant. When some diseases occur in the human body, the number of certain types of white blood cells will increase or decrease. In addition, some immature white blood cells with higher DNA content than normal white blood cells do not appear in the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/00G01N15/10
CPCG01N15/00G01N15/10G01N15/1031G01N2015/1006G01N2015/016G01N15/1023G01N2015/1028G01N2015/1029G01N2015/1024
Inventor 白红霞刘玲
Owner SONOSCAPE MEDICAL CORP
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