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Biochar-based microbial agent as well as preparation method and application thereof

A microbial agent and biochar technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of secondary pollution, biological and groundwater hazards, and high repair costs of chemical repair methods, achieving the same Less pollution, great application potential, and easy operation

Active Publication Date: 2020-04-10
AGRI ECOLOGY INST FUJIAN ACADEMY OF AGRI SCI
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Problems solved by technology

Once these zinc-containing wastewater enters the environment, it will cause immeasurable harm to surrounding organisms and groundwater
[0003] The treatment methods for zinc pollution mainly include physical and chemical methods such as chemical precipitation, ion exchange, adsorption, and membrane separation, and biological remediation methods such as microbial remediation. However, chemical remediation methods have high remediation costs and are prone to secondary pollution. and other defects

Method used

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  • Biochar-based microbial agent as well as preparation method and application thereof
  • Biochar-based microbial agent as well as preparation method and application thereof

Examples

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Embodiment 1

[0018] A preparation method of biochar-based microbial bacterial agent, the steps are as follows:

[0019] Step 1: organic waste such as wheat straw, corn straw, corn cob, peanut shell, bamboo (wood) chips, etc. 2 Under an inert gas atmosphere, pyrolyze at 350°C-700°C for 3-5h to produce biochar materials and pass through a 60-mesh sieve for later use;

[0020] Step 2: Prepare the seed solution of Ralstonia sp. (Ralstonia sp.) Ralstonia Bcul-1 (this Ralstonia sp. is isolated and purified from the farmland soil around Fujian Youxi lead-zinc mining area, and has heavy metal-resistant microorganisms The characteristic bacterial strain. The strain is preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, referred to as CGMCC, and the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing, and the preservation date is 2019.04 12, the deposi...

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Abstract

The invention relates to a microbial agent, in particular to a biochar-based microbial agent as well as a preparation method and application thereof. The biochar-based microbial agent comprises biochar and (Ralstonia sp.) Ralstonia bcul-1 adsorbed on the biochar. The preparation method comprises the following steps: (1), preparing a biochar-(Ralstonia sp.) Ralstonia bcul-1 mixed solution containing biochar, sodium alginate and a (Ralstonia sp.) Ralstonia bcul-1 seed solution, and performing standing overnight at a room temperature; (2), slowly dropwise adding the mixed solution into a calciumchloride solution with the mass concentration of 1%, continuously performing stirring, performing crosslinking to obtain a product, and cleaning the product with deionized water to obtain a microbialagent. The microbial agent is used for repairing heavy metal polluted water. According to the invention, zinc pollution is repaired by using physical adsorption and bioanalysis; the preparation methodhas advantages of simple operation, high remediation efficiency, no secondary pollution and the like; and the biochar-based microbial agent as well as a preparation method and application thereof have great application potential.

Description

technical field [0001] The invention relates to a microbial bacterial agent, in particular to a biochar-based microbial bacterial agent and its preparation method and application. Background technique [0002] With the massive discharge of industrial sewage, domestic wastewater and agricultural sewage, heavy metal pollution in water has become a global environmental pollution problem. Zinc pollution is one of the most common pollution. In agriculture, the application of zinc as a feed additive has good effects on the growth, development, immunity, reproduction and carcass quality of pigs, cattle, sheep, chickens, ducks and other livestock and poultry; but the utilization rate of zinc by livestock and poultry Low, for example, the absorption rate of zinc in adult monogastric animals is only 5%-10%, so that most of the zinc ingested is excreted with excrement, so that there is potential zinc pollution in livestock and poultry wastewater. In addition, industrial wastewater di...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/14C12N11/10C02F3/34C02F1/28C01B32/05C12R1/01C02F101/20
CPCC02F1/283C02F3/34C02F2101/20C12N1/20C12N11/10C12N11/14C01B32/05C12N1/205C12R2001/01
Inventor 王义祥黄家庆林怡叶菁李艳春刘岑薇
Owner AGRI ECOLOGY INST FUJIAN ACADEMY OF AGRI SCI
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