Novel cucumber downy mildew-resistant seedling culture substrate
A cucumber downy mildew and seedling raising substrate technology, applied in planting substrates, culture media, horticulture, etc., can solve the problems of long working cycle for disease-resistant breeding, pathogenic bacteria resistance, and environmental pollution, so as to reduce field incidence and ecological Significant benefits and simple production methods
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Embodiment 1
[0031] Example 1: Greenhouse Test
[0032] (1) Test method: 2 treatments and 3 repetitions were set up in the test. 20 plants were planted in each repetition, and 10 continuous plants were taken for investigation. The seedlings cultured on the matrix were used as the control group. When the cucumber seedlings grew to 3 leaves and 1 heart, they were transplanted into the nutrient pots with two kinds of substrates under greenhouse conditions. When 5 true leaves grew, they were sprayed with cucumber downy mildew disease pathogenic bacteria, investigate the disease severity and calculate the biocontrol effect after inoculating the pathogenic bacteria 20 days, disease severity=∑(number of diseased plants at all levels×level value) / (total number of plants under investigation×highest level value)×100%; control effect=( Severity of disease in the control group - severity of disease in the treatment group) / severity of disease in the control group × 100%.
[0033] (2) Grading standards...
Embodiment 2
[0045] Embodiment 2: field test
[0046] (1) Test method: The planting method is direct seeding, planting in large pots, and the substrates used are biocontrol substrates and conventional substrates. There are 3 treatments in the test, which are biocontrol substrates, chemical conventional substrates, and conventional substrates. , each treatment was repeated 3 times, and 18 pots were planted in each repetition. A random block design was adopted, and guard rows were set between plots. The field experiment adopted a standard field management model. In order to better test the effect of preventing cucumber downy mildew, the new seedling substrate and one group of conventional substrates were not sprayed with any pesticides during the preliminary investigation, and the other group of conventional substrates was controlled by chemical pesticides. Spray chemical pesticides once after each investigation; survey takes 10 continuous pots in each plot, and conducts the first investigat...
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