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Complex microbial inoculant for regulating balance of gynecological flora

A technology of compound bacterial agent and bacterial flora balance, applied in the field of microorganisms, can solve problems such as inconvenience and low efficiency, and achieve the effects of convenient consumption, broad prospects and exact ingredients.

Active Publication Date: 2020-04-07
射阳县康凯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although vaginal administration is direct and effective, it is not convenient enough, and many women have resistance
The prior art has confirmed that some of the lactic acid bacteria taken orally will enter the vagina, but the efficiency is low

Method used

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  • Complex microbial inoculant for regulating balance of gynecological flora
  • Complex microbial inoculant for regulating balance of gynecological flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Mice modeling method: 35 female mice weighing 20-22 g were divided into 7 groups, and there was no statistical difference among the mice in each group. The 7 groups were blank control group (normal mice), positive control group (levofloxacin group), negative control group, Lactobacillus crispatus CGMCC 12743 treatment group, Lactobacillus casei LC-N235 treatment group, Lactobacillus crispatus + Lactobacillus casei LC-N235 Treatment group, curly+Lactobacillus vulgaris casei treatment group. There were 5 mice in each group. Among them, the normal mouse group was used as a blank control group, and the positive control group, negative control group, and treatment group all used mice successfully modeled with bacterial vaginosis.

[0023] Bacterial vaginosis modeling method: Give mice a vaginal injection of 0.3 mL of mixed bacterial suspension prepared by E. 0.5*10 7 cfu / ml), after 7 days of continuous treatment, the vaginal opening of the mouse can be seen to have obviou...

Embodiment 2

[0033] After 7 days of treatment, the vaginal flora after each group of treatment in Example 1 was detected on the 8th day:

[0034] Detection of vaginal flora in mice: Take 100ul of vaginal douching solution under sterile conditions, mix it with normal saline, and then perform 10-fold serial dilutions in sequence, and select 10 times from the original solution. 3 、10 4 、10 5 、10 6Dilution, were dropped in MRS, EMB, SP, LB medium. After 48 hours of aerobic culture at 37°C (Lactobacillus was cultured anaerobically), the colonies were counted, and the number of live bacteria (cfu / ml), the logarithm was taken for statistical processing. The results are shown in Table 2.

[0035] Table 2 Detection of vaginal flora after treatment in different groups of mice

[0036]

[0037] Note: t test, l: P<0.05 (compared with blank group); 2: P<0.05 (compared with positive control group); 3: P<0.05 (compared with negative control group)

[0038] After corresponding treatment, the ch...

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Abstract

The invention discloses a complex microbial inoculant for regulating the balance of gynecological flora. The complex microbial inoculant comprises Lactobacillus crispatus, Lactobacillus casei LC-N235and other acceptable drug excipients. Preferably, the Lactobacillus crispatus is Lactobacillus crispatus CGMCC 12743. The Lactobacillus casei LC-N235 is preserved in the China General MicrobiologicalCulture Collection Center, and the preservation number is CGMCC 18057. The complex microbial inoculant can be made into an oral liquid or fermented food, preferably fermented milk, fermented soymilk and the like.

Description

technical field [0001] The invention relates to a compound microbial agent for regulating the balance of gynecological flora, and belongs to the technical field of microorganisms. Background technique [0002] A large number of bacteria live in the gastrointestinal tract of normal people. Newborns begin to have different bacteria entering the body 2 hours after birth, and then gradually establish a normal flora. There are more than 500 kinds of bacteria in the adult body, belonging to more than 50 species. More than 100 trillion. The intestinal flora is an extremely complex ecosystem composed of aerobic bacteria, anaerobic bacteria and facultative anaerobic bacteria. Under normal circumstances, they reproduce in the host without causing disease, and play a positive role in the health of the body. , including assisting digestion, absorption, nutrition, immunity and antagonizing pathogenic bacteria. Probiotics refer to those bacteria that have beneficial physiological activi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P15/02A61P31/04A23C9/123A23C11/06
CPCA61K35/747A61P15/02A61P31/04A23C9/1234A23C11/06A23V2400/125A61K2300/00
Inventor 叶水文张楠
Owner 射阳县康凯生物科技有限公司
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