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Polyurethane/decellularized fibrous ring matrix fibrous scaffold and preparation and application thereof

A technology of matrix fibers and annulus fibrosus, applied in medical science, tissue regeneration, prostheses, etc., to achieve high gene expression levels and light inflammatory reactions

Inactive Publication Date: 2020-03-31
THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no coaxial electrospun scaffolds of decellularized annulus fibromatrix (DAFM) and PECUU for AF tissue engineering have been reported.

Method used

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  • Polyurethane/decellularized fibrous ring matrix fibrous scaffold and preparation and application thereof
  • Polyurethane/decellularized fibrous ring matrix fibrous scaffold and preparation and application thereof
  • Polyurethane/decellularized fibrous ring matrix fibrous scaffold and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method of polyurethane / decellularized annulus fibrous matrix fiber scaffold, comprising the steps of:

[0039] Select 6-week-old New Zealand white rabbits, remove the nucleus pulposus, lateral fascia, and adipose tissue around the annulus fibrosus of the above-mentioned rabbits, rinse with phosphate buffered saline, cut into pieces and grind, then add trypsin at a concentration of 0.3% The solution was incubated for 23 hours at a temperature of 37°C, and the trypsin solution was replaced every 4 hours. After completion, it was washed with phosphate buffered saline, and then ribozyme solution (10mmol / L Tris-HCl, 50U / mL DNase and 1U / mL RNase, pH=7.5) was digested for 13h at a digestion temperature of 37°C, treated with 1% Triton X-100 for 23h, then washed with phosphate buffered saline, and the precipitate was dissolved in 3.5% acetic acid In the solution, store at 4°C to obtain the decellularized annulus fibrosus matrix;

[0040] The internal solution is o...

Embodiment 2

[0043] A preparation method of polyurethane / decellularized annulus fibrous matrix fiber scaffold, comprising the steps of:

[0044] Select 8-week-old New Zealand white rabbits, remove the nucleus pulposus, lateral fascia and adipose tissue around the annulus fibrosus of the above-mentioned rabbits, rinse with phosphate buffered saline, cut into pieces and grind, then add trypsin at a concentration of 0.2% The solution was incubated for 25 hours at a temperature of 37°C, and the trypsin solution was replaced every 4 hours. After completion, the solution was washed with phosphate buffered saline, and then ribozyme solution (10mmol / L Tris-HCl, 50U / mL DNase and 1U / mL RNase, pH=7.5) was digested for 11 hours at a digestion temperature of 37°C, treated with 1% Triton X-100 for 25 hours, then washed with phosphate buffered saline, and the precipitate was dissolved in 2.5% acetic acid In the solution, store at 4°C to obtain the decellularized annulus fibrosus matrix;

[0045] The int...

Embodiment 3

[0048] A preparation method of polyurethane / decellularized annulus fibrous matrix fiber scaffold, comprising the steps of:

[0049] Select 7-week-old New Zealand white rabbits, remove the nucleus pulposus, lateral fascia, and adipose tissue around the annulus fibrosus of the above-mentioned rabbits, wash with phosphate buffered saline three times, cut into small pieces of 1mm×1mm×1mm, and place Grind in a mortar, move to a constant temperature oscillator at 37°C, and then add trypsin solution with a concentration of 0.25% to incubate for 24 hours, start decellularization, and replace the trypsin solution every 4 hours;

[0050] After incubation, wash with phosphate-buffered saline for 3 times, then add ribozyme solution (10mmol / L Tris-HCl, 50U / mL DNase and 1U / mL RNase, pH=7.5), and place in a constant temperature shaker at 37°C. The tissue was further digested for 12 hours, treated with 1% Triton X-100 for 24 hours, and then washed 6 times with phosphate buffered saline, 8 hou...

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Abstract

The invention discloses a polyurethane / decellularized fibrous ring matrix fibrous scaffold, which has a core-shell structure. The polyurethane is used as a core and the decellularized fibrous ring matrix is used as a shell. Besides, the invention also discloses a preparation method of the polyurethane / acellular fibrous ring matrix fibrous scaffold. Polyurethane is used as a solute of an internal solution and a decellularized fibrous ring matrix is used as a solute of an external solution; coaxial electrostatic spinning is performed to obtain a polyurethane / acellular fibrous ring matrix fibrousscaffold. During the coaxial electrostatic spinning process, the flow velocity of the internal solution is 0.4 to 0.6 mL / h, the flow velocity of the external solution is 0.15 to 0.25 mL / h, the voltage of the mandrel is 15 to 17 kV, and the rotating speed of the mandrel is 180 to 220 rpm. In addition, the invention also discloses application of the polyurethane / acellular fibrous ring matrix fibrous scaffold in preparation of drugs for treating intervertebral disc degenerative diseases.

Description

technical field [0001] The invention relates to the technical field of biological tissue engineering, in particular to a polyurethane / decellular fibrous annulus matrix fiber scaffold and its preparation method and application. Background technique [0002] Low back pain caused by degenerative disc disease is one of the most common orthopedic diseases, with morbidity and disability rates that seriously affect the quality of life of patients. According to foreign reports, due to low back pain, more than 8 million Americans go to the clinic every year. The number of lost working days is as high as 89 million, and the medical costs and economic losses in the United States are as high as 90 billion US dollars per year. Therefore, the treatment of degenerative disc disease has huge economic and social benefits. At present, the effective treatment of this disease is mainly to reduce the pressure through surgery, but the effect of long-term follow-up is not clear, and it even caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/50
CPCA61L27/18A61L27/3633A61L27/3687A61L27/50A61L2430/38C08L75/04
Inventor 刘晨吴辰张玙
Owner THE FIRST AFFILIATED HOSPITAL OF WANNAN MEDICAL COLLEGE YIJISHAN HOSPITAL OF WANNAN MEDICAL COLLEGE
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