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Method suitable for manufacturing ovary paraffin sections of equus animals

A technology for paraffin sectioning and ovaries, applied in the field of biological research, can solve the problems of inapplicable equine animal ovary tissue, etc., and achieve excellent staining effect, promote exchange, and good staining effect

Pending Publication Date: 2020-03-27
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] To this end, the embodiment of the present invention provides a method suitable for making paraffin sections of ovaries of equine animals, so as to solve the existing problems in the prior art that are suitable for animal tissues due to the particularity of the ovarian tissue structure and properties of equine animals. The paraffin section preparation method is not applicable to the problem of equine ovarian tissue

Method used

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  • Method suitable for manufacturing ovary paraffin sections of equus animals
  • Method suitable for manufacturing ovary paraffin sections of equus animals
  • Method suitable for manufacturing ovary paraffin sections of equus animals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method suitable for making paraffin sections of equine ovaries comprises the following steps:

[0035] (1) Sampling fixed

[0036] The test animals of the genus equine were killed by carotid artery bloodletting, and then the ovaries were taken out, and the ovarian mesangium and ovarian medulla were cut off to obtain ovarian tissue. The ovarian tissue was fixed with fixative solution, packed in an ice bag box, and transported back to the laboratory for further examination. Transfer to a medical freezer at -4°C for 24 hours; the fixative is Carnot’s fixative; the Carnot’s fixative is made of glacial acetic acid: chloroform: absolute ethanol in a volume ratio of 1:3:6 made.

[0037] (2) Dehydration

[0038] Cut the sampled and fixed ovarian tissue longitudinally into tissue fragments with a thickness of 0.3-0.6 cm, and pass the cut tissue fragments through alcohol with a concentration of 75%, alcohol with a concentration of 85%, alcohol with a concentration of 95%, and...

Embodiment 2

[0048] The technical solution of this embodiment is compared with embodiment 1, except that the pumping pressure in step (5) is 0.02MPa, other technical solutions are the same as embodiment 1.

Embodiment 3

[0050] The technical solution of the present embodiment is compared with embodiment 1, except that the pumping time in step (5) is 40min, other technical solutions are the same as embodiment 1.

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Abstract

The embodiment of the invention discloses a method suitable for manufacturing ovarian paraffin sections of equus animals, and relates to the technical field of biological research. The method comprises the following steps: sampling and fixing, dehydrating, transparentizing, waxing, exhausting, embedding and section manufacturing. According to the method suitable for manufacturing the ovary paraffin sections of the equus animals, the air exhaust process is added; bubbles existing in ovarian tissues of equus animals in the waxing process are effectively removed; the ovary paraffin section of equus animals prepared by the method can effectively protect the integrity of the tissue structure of the ovary of equus animals, and can obtain a good staining effect, and the integrity of follicles canbe maintained to a great extent, so that good paraffin dipping and embedding effects are obtained.

Description

technical field [0001] The embodiment of the present invention relates to the technical field of biological research, in particular to a method suitable for making paraffin sections of ovaries of equine animals. Background technique [0002] Paraffin section is the most basic method of making slices in histological observation. As a traditional biological research technique, paraffin section can not only observe the morphology of normal tissue cells, but also can be used in pathology, immunology, and cell in situ. Nucleic acid molecular hybridization and morphometric fields are also widely used. Paraffin sections need to go through the steps of fixation, dehydration, transparency, paraffinization, embedding, sectioning, patching, dewaxing, staining, transparency, and mounting to maintain the original structure of tissue cells and allow them to be clearly observed and analyzed. [0003] Because the structure and properties of the ovaries of equine animals are very different ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/36G01N1/28G01N1/04
CPCG01N1/30G01N1/36G01N1/286G01N1/04G01N2001/302G01N2001/2873
Inventor 格日乐其木格神英超陶力王希生白东义赵一萍张心壮李蓓芒来
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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