Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Idiopathic pulmonary fibrosis disease diagnosis marker and application thereof in preparing diagnosis or prognosis tool

A technology for pulmonary fibrosis and disease diagnosis, which is applied in the field of medical diagnosis and can solve the problems of lack of specificity, confusing diagnosis, and unclear etiology.

Pending Publication Date: 2020-03-27
HENAN NORMAL UNIV
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The etiology of idiopathic pulmonary fibrosis is unclear and the diagnosis is easily confused. The clinical methods used to diagnose idiopathic pulmonary fibrosis often lack specificity. More efficient and stable methods are urgently needed to warn or judge idiopathic pulmonary fibrosis. pulmonary fibrosis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Idiopathic pulmonary fibrosis disease diagnosis marker and application thereof in preparing diagnosis or prognosis tool
  • Idiopathic pulmonary fibrosis disease diagnosis marker and application thereof in preparing diagnosis or prognosis tool
  • Idiopathic pulmonary fibrosis disease diagnosis marker and application thereof in preparing diagnosis or prognosis tool

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Screening for genes differentially expressed in patients with idiopathic pulmonary fibrosis and normal controls

[0059] 1. Clinical research objects:

[0060] A total of 30 patients with idiopathic pulmonary fibrosis were selected, including 21 males and 9 females, with an age range of 30-83 years old. The diagnostic criteria were in accordance with the "Special Pulmonary Disease" revised by the Chinese Respiratory Society for Interstitial Lung Disease and the American Thoracic Society (ATS). Guidelines for the diagnosis and treatment of paroxysmal pulmonary fibrosis.

[0061]Exclusion criteria: (1) Patients with other lung diseases, such as bronchial asthma, pulmonary interstitial fibrosis, lung cancer, etc.; (2) Patients with infection in other parts; (3) Patients with severe cardiovascular and cerebrovascular diseases, diabetes, blood system Diseases, malignant tumors, organ failure, and hepatitis; (4) Those who suffer from immune system diseases or have recently u...

Embodiment 2

[0070] Validation of differentially expressed genes in idiopathic pulmonary fibrosis patients and normal controls

[0071] 4. Detection of COX5B by ELISA

[0072] Example 2 Verification of COX5B differentially expressed in idiopathic pulmonary fibrosis patients and normal subjects.

[0073] 5. Blood total peptide extraction, mass spectrometry detection

[0074] Using LC-MS / MS mass spectrometry Example 2 to verify the content of COX5B in the blood of patients with idiopathic pulmonary fibrosis and normal people.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an idiopathic pulmonary fibrosis disease diagnosis marker and application thereof in preparing a diagnosis or prognosis tool. The invention relates to novel discovery of a cytochrome C oxidase subunit 5B (COX5B) as an idiopathic pulmonary fibrosis (IPF) patient serum marker. COX5B is one of constitution components of respiratory chain composites III on a mitochondrial innermembrane, the content of COX5B in IPF patient serum is increased, it means that mitochondria of an IPF patient is seriously damaged, COX5B is released into the serum, in addition, the content of COX5B in the serum of the IPF patient is in negative correlation with Oversall Survival, and the content of COX5B in serum of an IPF patient in poor survival is high. Research results disclosed by the invention provide a noninvasive method for clinical diagnosis on idiopathic pulmonary fibrosis diseases and are applicable to clinical popularization.

Description

technical field [0001] The invention belongs to the technical field of medical diagnosis, and in particular relates to a diagnostic marker for idiopathic pulmonary fibrosis and its application in the preparation of diagnostic or prognostic tools. Background technique [0002] Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, lethal disease characterized pathologically by destruction of the lung parenchyma, marked changes in alveolar epithelial cell phenotype, deposition of extracellular matrix, and abnormal proliferation and accumulation of fibroblasts. There are now more than 200 known causes of idiopathic pulmonary fibrosis, such as genetic diseases, autoimmune diseases, exposure to toxic substances in the environment, drugs, and radiation. More and more evidence shows that in the development of pulmonary fibrosis, not only inter-heterogeneity and intra-heterogeneity exist widely, but also extensive There are dynamic changes in the individual at various level...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68G01N33/543C07K14/47C12N15/12
CPCC12Q1/6883G01N33/6884G01N33/54306C07K14/47C12Q2600/118C12Q2600/158G01N2800/12G01N2333/47
Inventor 王兰余国营
Owner HENAN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com