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Method for detecting smoking addiction of Chinese Han population by single nucleotide polymorphism

A single nucleotide polymorphism and addiction technology, applied in the field of biogenetic medicine, can solve problems such as loss of heritability and loss of heritability

Pending Publication Date: 2020-03-24
刘强 +1
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  • Application Information

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Problems solved by technology

However, such a single analysis caused the loss of heritability, and many loci that were not significant enough were ignored. This phenomenon also explained the phenomenon of heritability loss.

Method used

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  • Method for detecting smoking addiction of Chinese Han population by single nucleotide polymorphism
  • Method for detecting smoking addiction of Chinese Han population by single nucleotide polymorphism
  • Method for detecting smoking addiction of Chinese Han population by single nucleotide polymorphism

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Example 1: Screening susceptibility loci for smoking addiction based on related research and customizing Taqman OpenArray:

[0028] Based on domestic and foreign peer-reviewed journal papers and the results of our team's previous work, we screened the genes of the nicotine receptor family (Nicotinic receptor subunit and other cholinergic system genes), genes related to nicotine metabolism (Nicotine metabolism genes), neurotransmitters Related genes in the system (Neurotransmitter system genes), a total of 18 candidate genes and 203 single nucleotide polymorphism sites were used as research targets (see Table 1 for details).

[0029] 1) 18 candidate genes related to addiction;

[0030] 2) SNPs of 203 candidate genes (Table 1);

[0031] 3) Customize the Taqman OpenArray chip, and select the optimal module combination according to the number of samples and the number of single nucleotide polymorphism sites.

Embodiment 2

[0032] Example 2: Results of genotyping, transcriptome sequencing, and methylation sequencing:

[0033] After completing the genotyping experiment, use TaqMan Genotyper (v.3.3) to analyze and check the quality of the off-machine data. Using the "Autocall" function to call all SNPs, this process requires two independent researchers to manually check the results of the automatic call to ensure the accuracy of the results. According to the results of genotyping, that is, wild homozygous type, homozygous mutant type and heterozygous type, it is judged whether a person carries smoking addiction susceptibility loci.

[0034] For the off-machine data of transcriptome sequencing and methylation sequencing, the quality control of the original data should be carried out first to remove low-quality samples and sequences, so as to avoid the impact of low-quality results on the analysis. Afterwards, the individual samples were combined with references, and the genes were assembled and qua...

Embodiment 3

[0035] Example 3: Based on the results of genotyping, genome methylation sequencing, and transcriptome sequencing, using cis-mQTL analysis and cis-eQTL analysis, a second study of the unique smoking addiction susceptibility loci in the Han population Screening to identify single nucleotide polymorphism sites with potential biological functions, and use bioinformatics software Polyphen and CADD and other bioinformatics analysis techniques to analyze the included single nucleotide polymorphism sites Scoring and retaining the loci that are most likely to contribute to the phenotype of smoking constitutes a genetic testing kit that can accurately distinguish smoking addiction in the Chinese Han population, and is used to accurately determine whether they are susceptible individuals to smoking addiction, thereby avoiding Direct and excessive exposure to tobacco products, to achieve the purpose of precise prevention, and to find a detection kit for smoking addiction sites unique to t...

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Abstract

The invention discloses a method for detecting smoking addiction of the Chinese Han population by single nucleotide polymorphism, and the method comprises the following steps: 1, determining 18 susceptibility genes and 203 single nucleotide polymorphism sites related to smoking addiction, and customizing a Taqman OpenArray chip for genotyping; 2, extracting DNA and RNA from a blood sample; 3, carrying out preliminary screening on specific smoking addiction susceptibility sites in the Chinese Han population; 4, based on the results of genotyping, genome methylation sequencing and transcriptomesequencing, finding out a single nucleotide polymorphism site with a potential biological function. The invention relates to the technical field of biogenetic medicine. According to the invention, themethod can provide basis and guidance accurately and effectively for judging the smoking addiction susceptibility of the Chinese Han population, so that high-risk individuals are prevented from directly or excessively contacting tobacco products, and the purpose of accurate prevention is achieved.

Description

technical field [0001] The invention relates to the technical field of biogenetic medicine, in particular to a detection kit for a single nucleotide polymorphism site of a smoking addiction susceptibility gene of a Chinese Han population and an application thereof. Background technique [0002] Smoking addiction, as a chronic mental disease, causes about 6 million abnormal deaths every year worldwide. Due to the lack of effective treatment methods and smoking cessation methods, as well as the limited awareness of the hazards of smoking addiction in some countries and regions, the number of abnormal deaths caused by smoking will reach 10 million per year in 2020. According to the report of the China Center for Disease Control and Prevention, in 2015, the proportion of adult males who had smoking experience in China was as high as 52.1%, the total number of smokers exceeded 300 million, and 60 million people smoked more than 1 pack a day. 1.3 million. The economic loss cause...

Claims

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Application Information

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IPC IPC(8): G16B20/20G16B20/40C12Q1/6883
CPCG16B20/20G16B20/40C12Q1/6883C12Q2600/156
Inventor 刘强马云龙李明定
Owner 刘强
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