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A method for the biological removal of tetracycline antibiotics

A technology of tetracyclines and antibiotics, applied in the field of environmental remediation, can solve the problems of tetracycline antibiotics that have not been removed, and achieve the effects of improving biological removal efficiency, eliminating separation and purification, and reducing biological toxicity

Active Publication Date: 2022-04-29
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the biological removal of tetracycline antibiotics by the mixed fermentation of Bacillus clausii and bacteria that can produce surfactants

Method used

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  • A method for the biological removal of tetracycline antibiotics
  • A method for the biological removal of tetracycline antibiotics
  • A method for the biological removal of tetracycline antibiotics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The first seed medium, tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L, the balance is water.

[0032] The second seed medium, glucose 60g / L, beef extract 10g / L, peptone 10g / L, yeast extract 10g / L, NaCl 5g / L, and the balance is water.

Embodiment 2

[0034] The formula of the fermentation medium is: glucose 55g / L; yeast extract powder 8g / L; peptone 8g / L; NaCl 8g / L; KCl 0.8g / L; K 2 HPO 4 5 g / L; KH 2 PO 4 5 g / L; MgSO 4 ·7H 2 O 0.8g / L; FeSO 4 ·7H 2 O 1.5×10 -3 g / L; CuSO 4 ·5H 2 O 1.5×10 -5 g / L; MnSO 4 8×10 -5 g / L; ZnSO 4 ·7H 2 O 1.5×10 -3 g / L; the rest is water.

Embodiment 3

[0036] The formula of the fermentation medium is: glucose 65g / L; yeast extract powder 3g / L; peptone 3g / L; NaCl 3g / L; KCl 0.3g / L; 2 HPO 4 1 g / L; KH 2 PO 4 1 g / L; MgSO 4 ·7H 2 O 0.3g / L; FeSO 4 ·7H 2 O 0.5×10 -3 g / L; CuSO 4 ·5H 2 O 0.5×10 -5 g / L; MnSO 4 5×10 -5 g / L; ZnSO 4 ·7H 2 O 0.5×10 -3 g / L; the rest is water.

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Abstract

The invention discloses a method for biologically removing tetracycline antibiotics, comprising the following steps: (1) seed cultivation: culturing secondary seeds of Bacillus clausii and Bacillus amyloliquefaciens; (2) removing: removing tetracycline antibiotics The degraded water is mixed with the fermentation medium to form a degradation solution, and the secondary seeds of Bacillus clausii and the secondary seeds of HM-618 are inserted into the degradation solution for fermentation. The invention establishes a co-cultivation system of Bacillus clausii and Bacillus amyloliquefaciens, and adds them to the water body containing tetracycline antibiotics, so that Bacillus amyloliquefaciens in situ produces surface active substances, promotes the biological removal of tetracycline antibiotics, saves money A series of tedious steps such as separation and purification of surfactants are eliminated; the invention not only improves the biological removal efficiency of tetracycline antibiotics, but also significantly reduces the biological toxicity of tetracycline antibiotics transformation products.

Description

technical field [0001] The invention belongs to the field of environmental restoration and relates to a method for biologically removing tetracycline antibiotics assisted by surfactants. Background technique [0002] Tetracycline antibiotics represented by oxytetracycline (OTC) and chlorotetracycline (CTC) are a class of broad-spectrum antibiotics, widely used in animal husbandry and aquaculture, and their usage accounts for 14% of the total antibiotic usage. Tetracycline antibiotics were detected in all types of water environment. It not only leads to serious water pollution, threatens the survival of aquatic plants, animals and microorganisms, but also leads to the emergence of tetracycline resistance in bacteria and the spread of resistance genes. Therefore, it is urgent to find effective ways to remove tetracycline antibiotics from water . [0003] Traditional physical methods such as adsorption and photocatalysis are costly and easily cause secondary pollution. Many ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/38
CPCC12N1/20C02F3/341C02F2101/38
Inventor 程景胜刘晓春元英进
Owner TIANJIN UNIV
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