Multi-effect eye repairing essence and preparation method thereof
An essence and eye technology, applied in skin care preparations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of eliminating dark circles, diluting fine lines, and ineffective repairing effects. Problems such as the formation of fat granules in the eye area, achieve the effect of moisturizing, strengthening the elastic network around the eyes, inhibiting proliferation and maturation
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Embodiment 1
[0027] The preparation of embodiment 1 Cimicifuga stem cell extract
[0028] The preparation of Cimicifuga stem cell extract comprises the following steps:
[0029] S1: Take the tuber root containing the cambium of Cimicifuga as explants, soak it with 70% ethanol solution for 5 minutes on the ultra-clean workbench, then rinse it with sterile water for 3 to 5 times, and dry the surface moisture with sterile filter paper , cut into small pieces of 0.3cm×0.3cm, inoculated in the medium of MS+0.1mg / L NAA+2mg / L BA+60g / L sucrose+6g / L agar, cultured in the dark at 25±2℃ for 25 day, the cambium cells were separated from other tissue cells to obtain cambium cells;
[0030] S2: Get the cambium cells with good growth state obtained in S1, inoculate them into the subculture medium and carry out shaker dark culture, the subculture medium is MS+0.1mg / L NAA+2mg / L BA+30g / L sucrose, Culture conditions: temperature 30°C, culture speed 120r / min, culture for 4 weeks;
[0031] S3: Subsequent ce...
Embodiment 2
[0033] The preparation of embodiment 2 Cimicifuga stem cell extract
[0034] The preparation of Cimicifuga stem cell extract comprises the following steps:
[0035] S1: Take the tuber root containing the cambium of Cimicifuga as explants, soak it with 70% ethanol solution for 5 minutes on the ultra-clean workbench, then rinse it with sterile water for 3 to 5 times, and dry the surface moisture with sterile filter paper , cut into small pieces of 0.3cm×0.3cm, inoculated in the medium of MS+2mg / L NAA+0.1mg / L BA+30g / L sucrose+7g / L agar, cultured in the dark at 25±2℃ for 25 day, the cambium cells were separated from other tissue cells to obtain cambium cells;
[0036] S2: Get the cambium cells with good growth state obtained in S1, inoculate them into the subculture medium and carry out shaker dark culture, the subculture medium is MS+2mg / L NAA+0.1mg / L BA+60g / L sucrose, Culture conditions: temperature 30°C, culture speed 120r / min, culture for 4 weeks;
[0037] S3: Subsequent ce...
Embodiment 3
[0039] The preparation of embodiment 3 multi-effect repair eye essence
[0040] The preparation of embodiment 3 multi-effect repairing eye essence liquid comprises the component of weight portion shown in the table below:
[0041]
[0042] Preparation steps: Mix the small molecule moisturizer, carboxymethyl β-glucan sodium, betaine and deionized water in the prescribed amount, heat to 70°C, stir to make it completely dissolve, cool down to 40°C, add undecyl Acenoyl phenylalanine, Cimicifuga stem cell extract, Wing algae extract, Quinoa extract, EDTA-disodium, phenoxyethanol and ethylhexylglycerin, mix evenly, and the essence is obtained after discharging.
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