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Application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicus breeding

A technology for predatory organisms and tea saponin, applied in the field of aquaculture, can solve the problems of adverse effects on the ecological environment and product safety, cumbersome processing technology, little progress, etc. High killing efficiency

Inactive Publication Date: 2020-03-13
MARINE BIOLOGY INST OF SHANDONG PROVINCE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the Ciona that has entered the nursery pond cultivation system and started to multiply, it can only be removed by manual sorting, which is not only time-consuming and laborious, but also soaked with chemical agents to prevent excessive stress or disease occurrence of the ginseng seedlings after sorting, which is harmful to the ecological environment. and product safety will have adverse effects; or use drugs to kill Ciona, but currently there are not many drugs that can effectively kill Ciona and have no adverse effects on sea cucumbers. There are still very few technical researches, and most of them focus on finding suitable killing drugs. For many years, aquatic disease control personnel have been exploring drugs to remove Ciona, but the progress is not great. Among them, there are reports of using Chinese herbal medicine compound to kill, However, there are problems such as complex drug formula, long dipping time, cumbersome post-treatment process, etc., and it has not been widely promoted and used in production.

Method used

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  • Application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicus breeding
  • Application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicus breeding
  • Application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicus breeding

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Qingdao National Marine Science Research Center Aquatic Seedling Industrialization Base Experiment

[0044] (1) The experimental container is a 60L sorting box. Each box is put into a corrugated basket with 50 glass ascidians and 30 ginseng seedlings. Oxygenation, water temperature 21.2 ℃, salinity 33.

[0045] (2) Set up four tea saponin concentration groups of 30ppm, 40ppm, 50ppm, and 60ppm respectively, and set up three parallel groups for each group.

[0046] (3) The ginseng seedlings were adapted to the medicated bath for 48 hours, stopped eating 1 day before the medicated bath, and stopped oxygenation during the medicated bath.

[0047] (4) After the medicated bath, the water outlet holes of the Ciona opened, and after death, the rucksack was wrinkled, inelastic, unresponsive to artificial stimulation, or attached to the board or fell off to the bottom of the box. The vitality of the ginseng seedlings in each group was normal, the spines did not shrink, and the ...

Embodiment 2

[0052] Experiment of a sea cucumber breeding company in Hongdao

[0053] (1) The implementation site is 3 cement cultivation ponds, and the size of the cement pond is 15m 3 / pond, the size of the ginseng seedlings cultured in the pond is 5g / head to 25g / head, and the diameter of the epiphytic Ciona is mostly greater than 5mm. Oxygenation is performed for 24 hours, the water temperature is 23.4°C, and the salinity is 32.

[0054] (2) Set three tea saponin concentrations of 40ppm, 50ppm and 60ppm respectively.

[0055] (3) Dissolve the tea saponin powder in seawater 2-3 days before use, filter it through a 200-mesh sieve, and put it in the refrigerator for later use.

[0056] (4) Splash the whole pool at 7 o'clock in the morning on the day of the medicinal bath, and pour the pool after 2 hours of stopping gas. The killing rates of Ciona in the three cultivation pools were 98%, 100%, and 100%, respectively.

[0057] (5) After changing the water and pouring the pool, sprinkle the ...

Embodiment 3

[0059] Experiment of a sea cucumber breeding company in Penglai

[0060] (1) The test site is 3 cement cultivation ponds, and the size of the cement pond is 20m 3 / pool, the size of japonicus seedlings in the pool is 1g / head to 20g / head, and the diameter of the epiphytic Ciona is mostly less than 5mm. Oxygenation is carried out for 24 hours. The water temperature is 22.8°C and the salinity is 33.

[0061] (2) Set three tea saponin concentrations of 30ppm, 40ppm and 50ppm respectively.

[0062] (3) Dissolve the tea saponin powder in seawater 2-3 days before use, filter it through a 200-mesh sieve, and put it in the refrigerator for later use.

[0063] (4) Splash the whole pond at 9 o'clock in the morning on the day of the medicinal bath, and pour the pond after stopping the gas for 3 hours. The killing rates of Ciona in the three cultivation ponds were 99%, 100%, and 100% respectively.

[0064] (5) After changing the water and pouring the pool, sprinkle the ginseng seedlings...

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Abstract

The invention provides an application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicas breeding, and belongs to the technical field of aquaculture. The application of tea saponin in killing of aquatic harmful organisms in Apostichopus japonicas breeding solves the problems of large extra consumption of manpower, material resources and time cost, influence on normalproduction planning and easy stimuli and physical damage to young Apostichopus japonicas in the operation process of a manual hand picking method in the prior art, and also solves the problems of complex medicine formula, long medicated bath time and complex medicated bath post-treatment process in the killing process of Chinese herbal medicine compound treatment. The tea saponin has the characteristics of high killing efficiency, thorough effect, no extra labor or time cost, no side effects on Apostichopus japonicas, and easiness in large-scale promotion and application.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of tea saponin in killing aquatic predators in sea cucumber cultivation. Background technique [0002] Sea squirts, copepods, shield ciliates and other marine organisms are the main predators in aquaculture. These organisms reproduce in large numbers, not only competing with sea cucumbers for living space and bait, but also consuming a large amount of dissolved oxygen and excreting metabolites into the water, thereby inhibiting the growth of aquatic animals. Taking Ciona as an example, Ciona is the main harmful organism in the japonicus seedling cultivation pond, and has strong reproductive ability. So far, there is no effective method to control and eliminate the large population of Ciona. At present, the method usually used in production is to eliminate Ciona through physical filtration. This has a certain effect on the production of nursery pon...

Claims

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Application Information

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IPC IPC(8): A01N55/10A01P15/00A01K61/13
CPCA01K61/13A01N55/00Y02A40/81
Inventor 韩莎李成林胡炜赵斌刘兆存解秀春
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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