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Sample pretreatment solution for quantitative vitamin D detection and use method of solution

A quantitative detection and vitamin technology, applied in the field of sample pretreatment solution for the quantitative detection of vitamin D, can solve the problems of unpredictable matrix effects and inaccurate quantification, and achieve the effect of reducing non-specific interference and improving accuracy.

Inactive Publication Date: 2020-02-28
重庆黄嘉同怡科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Matrix effects are often difficult to predict and can lead to inaccurate quantification if not handled properly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0022] A sample pretreatment solution for quantitative detection of vitamin D. Take 10ml of normal saline with a concentration of 0.9%. Based on normal saline, add 800mg of dextran sulfate sodium salt and 15mg of β-heterotropic blocking agent, and mix well After mixing 100 μL pretreatment solution with 200 μL serum sample, vortex and mix for 1 min, let it stand for 5 min, then put the mixed solution into a centrifuge, and centrifuge at 13000 rpm and 4 °C for 10 min. After centrifugation, take the solution Add 200 μL of ethanol to the supernatant, vortex and mix for 1 min, then let stand for 5 min, centrifuge the mixed solution at 13,000 rpm and 4°C for 10 min, take the supernatant into a new centrifuge tube, and use nitrogen gas Dry it with a blower, then add the separation mobile phase to reconstitute and mix well before it can be used for LC-MS / MS detection. This detection method can fully separate 25-hydroxyvitamin D from the binding protein. During the further separation an...

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PUM

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Abstract

The invention discloses a sample pretreatment solution for quantitative vitamin D detection. The solution comprises normal saline, dextran sulfate sodium salt and a beta-isotropism blocking agent. Thedextran sulfate sodium salt and the beta-isotropism blocking agent can be used to fully separate 25-hydroxyvitamin D to be analyzed from binding protein to a certain extent. Non-specific interferenceeffect in a serum matrix is reduced, the sample can be well separated in the further separation and purification process, a high-quality to-be-analyzed object can be obtained from the treated sample,and the quantitative detection accuracy of the vitamin D is improved.

Description

technical field [0001] The invention relates to the field of quantitative detection of vitamin D, in particular to a sample pretreatment solution for quantitative detection of vitamin D and a use method thereof. Background technique [0002] Vitamin D is a steroid hormone, including 2 types: vitamin D2 and vitamin D3. It is converted into 25-hydroxyvitamin D2 [25-hydroxyvitamin D2, 25 (OH) D2] and 25-hydroxyvitamin D3 [25-hydroxyvitamin D3, 25 (OH) D3] in the body through absorption, collectively referred to as 25-hydroxyvitamin D [ 25-hydroxyvitamin D, 25(OH)D]. 25(OH)D in the systemic circulation of the body is considered to be an important indicator reflecting the level of vitamin D in the human body. [0003] Vitamin D is an important trace element that participates in the body's physiological functions. The main physiological functions are to participate in the body's calcium and phosphorus metabolism and immune regulation. Patients with osteoporosis, osteomalacia, r...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027G01N2030/062
Inventor 王伟
Owner 重庆黄嘉同怡科技有限公司
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