Detection reagent for N-acetyl-beta-D-glucosidase
A technology of glucosamine and detection reagent, which is applied in the measurement of color/spectral characteristics, material analysis by observing the effect on chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of poor stability and sensitivity, etc. problem, to achieve the effect of improving strong alkaline environment, improving sensitivity and ensuring efficiency
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Embodiment 1
[0030] A traditional PNP-NAG method detection reagent, the composition of described reagent R1 and reagent R2 is as follows:
[0031] R1 reagent:
[0032] Glycine buffer 30mmol / L
[0033] p-Nitrophenyl-β-D-glucosamine 16mmmol / L
[0035] R2 reagent:
[0036] Borax buffer at pH 10.2 100mmol / L
Embodiment 2
[0039] NAG detection reagent of the present invention comprises reagent R1 and reagent R2, and the composition of described reagent R1 and reagent R2 is as follows:
[0040] R1 reagent composition:
[0041]
[0042] R2 reagent composition:
[0043] Sodium carbonate buffer 10~100mmol / L
[0044] Triton X-100 3mL / L~5mL / L
[0045] Preservatives 2g / L~5g / L.
[0046] The citric acid buffer solution is a citric acid buffer solution with a pH of 4-5 at 25°C.
[0047] The stabilizer is selected from one of EDTA, EDTA-2Na, ethylene glycol, and iminodiacetic acid.
[0048] The preservative is selected from one of NaN3, methyl p-hydroxybenzoate and 4-chloro-3,5-dimethylphenol.
[0049] Respectively, the reagents obtained in Example 1 and Example 2 were detected and used in a fully automatic biochemical analyzer with dual reagent functions, and determined by the rate method.
[0050] Detection and use method: place reagents R1 and R2 on the corresponding reagent positions according...
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