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Application of piRNA-26131 in preparation of thyroid cancer diagnostic and prognostic marker

A technology of pirna-26131, 1. pirna-26131, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve the problems of lack of thyroid cancer, tumors, genome destructive disasters, etc.

Active Publication Date: 2020-02-21
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the rotor sequence accounts for nearly 40% of the genome, and once this "jumping genes" is confused, it will lead to a devastating disaster in the genome and lead to the occurrence of tumors
However, there is a lack of reports on thyroid cancer and related piRNAs

Method used

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  • Application of piRNA-26131 in preparation of thyroid cancer diagnostic and prognostic marker
  • Application of piRNA-26131 in preparation of thyroid cancer diagnostic and prognostic marker
  • Application of piRNA-26131 in preparation of thyroid cancer diagnostic and prognostic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] In this example, piRNAs differentially expressed in thyroid cancer tissues and matched paracancerous tissue samples were screened by high-throughput sequencing. Among them, all thyroid cancer and matched paracancerous tissues were collected from the Department of Pathology, Shanghai Tenth People's Hospital, and confirmed by pathological examination. The patients informed and agreed that the samples were used in this study, and this study was approved and implemented by the Ethical Science Committee of Tongji University.

[0037] Depend on figure 1 It can be seen that the expression levels of 23 piRNAs were significantly down-regulated, and 5 piRNAs were significantly highly expressed in thyroid cancer tissues, including piRNA-26131. Therefore, the difference in expression of piRNA-26131 in thyroid cancer and its paired adjacent tissues was detected by qPCR method, as well as the difference in the expression level in blood of normal volunteers, preoperative blood of pat...

Embodiment 2

[0069] This example verifies the influence of piRNA-26131 on the metastasis of thyroid cancer cells.

[0070] Specifically include the following steps:

[0071] (1) Thyroid cancer cell culture: TPC1 cells were cultured in RPMI1640 medium containing 10% fetal bovine serum, BCPAP cells were cultured in RPMI1640 medium containing 10% fetal bovine serum and NEAA, and normal thyroid cells were cultured in RPMI1640 medium containing 10% fetal bovine serum All cells were incubated in DMEM medium with fetal bovine serum at 37°C, 5% CO 2 , Cultured in a saturated humidity cell constant temperature incubator.

[0072] (2) CCK8 proliferation test: Thyroid cancer cells were transiently transfected with piRNA-26131 mimics and inhibitors for 24 hours before CCK8 test: pour out the old medium, rinse once with PBS; add an appropriate amount of 0.25% trypsin, 37 After digestion at ℃ until the cells retract and become rounded or the intercellular space increases, immediately add complete medi...

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Abstract

The invention provides an application of piRNA-26131 in preparation of a thyroid cancer diagnostic and prognostic marker. The invention also provides an application of the piRNA-26131 in preparation of a kit for diagnosing a thyroid cancer or judging whether the thyroid cancer recurs. In addition, the invention provides a method for detecting the piRNA-26131 in thyroid cancer tissue or blood for non-diagnostic and therapeutic purposes. After overexpression or knockdown of the piRNA-26131, the proliferation and invasion metastasis ability of thyroid cancer cells are significantly enhanced or weakened, patients with piRNA-26131 high expression have high probability of recurrence, so that the piRNA-26131 can be used as the blood screen molecular marker for early screening and tumor recurrenceindicators of the thyroid cancer patients, and has great significance and application prospects.

Description

technical field [0001] The present invention relates to the field of biomedicine, in particular to the application of piRNA-26131 in the preparation of markers for diagnosis and prognosis of thyroid cancer. Background technique [0002] Thyroid cancer is one of the most common malignant tumors of the endocrine system, accounting for about 2% of all malignant tumors in the whole body, and it mostly occurs in young adults around 40 years old. Statistics show that the incidence of thyroid cancer is increasing year by year. At present, the early diagnosis of thyroid cancer still uses limited inspection methods such as physical examination and imaging, which have certain limitations and lack early warning value. Commonly used diagnostic markers are easily confused with thyroiditis and thyroid nodules, thus easily causing misdiagnosis. With the continuous advancement of molecular biology techniques, it is imperative to develop new blood tumor markers in order to meet the requirem...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 蔚青常争艳黄莉莉高耀辉
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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