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Bacillus paramycoides fermentation method

A technology of Bacillus mycoides and fermentation methods, applied in the field of bioengineering, can solve problems such as waste of resources, pollution of the environment, complex processes, etc., and achieve the effect of solving difficult problems

Active Publication Date: 2020-02-18
GUANGXI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent years, my country's poultry breeding industry has developed rapidly. Feathers can account for 5%-7% of the weight of broilers, and the annual output exceeds 1 million tons. However, most of them are not utilized and are usually landfilled or incinerated. Waste, and seriously pollute the environment, and even spread diseases, causing Newcastle disease, air bag inflammation and keratoconjunctivitis. health problems
At present, physical and chemical methods such as high temperature and high pressure, strong acid and strong alkali, and extrusion are usually used to treat feathers. The process is complicated, consumes a lot of energy, and is easy to cause environmental pollution.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] like Figure 1 to Figure 2 Shown, technical scheme of the present invention is: the material that the present invention adopts and experimental method comprise the following steps:

[0034] A method for fermenting Bacillus pseudomycoides, comprising the following steps:

[0035] (a) Making the fermentation base

[0036] Feathers are sterilized, and a culture solution is added in a certain proportion to obtain a fermentation bottom material; the culture solution contains a carbon source, and each liter of the culture solution contains 2 g of a carbon source, and the carbon source is glucose; the culture solution Contains a nitrogen source, 2g of a nitrogen source per liter of the culture solution, and the nitrogen source is beef powder; the culture solution also contains an inorganic salt, 0.05g of an inorganic salt per liter of the culture solution, and the inorganic salt The salt is anhydrous magnesium sulfate.

[0037] (b) Inoculate with bacteria that can degrade f...

Embodiment 2

[0044] like figure 1 , image 3 , Figure 5 and Image 6 Shown, technical scheme of the present invention is: the material that the present invention adopts and experimental method comprise the following steps:

[0045] A method for fermenting Bacillus pseudomycoides, comprising the following steps:

[0046] (a) Making the fermentation base

[0047] Feathers are sterilized by adding a culture solution in a certain proportion to obtain a fermentation base; the culture solution contains a carbon source, and every liter of the culture solution contains 10 g of a carbon source, and the carbon source is soluble starch; the culture solution contains Nitrogen source, containing nitrogen source 10g in every liter of described culture fluid, described nitrogen source yeast powder; Also contain inorganic salt in the described culture fluid, contain inorganic salt 0.2g in the described culture fluid of every liter, described inorganic salt is copper sulfate.

[0048] (b) Inoculate ...

Embodiment 3

[0055] like figure 1 and Figure 4 Shown, a kind of Bacillus mycoides fermentation method comprises the following steps:

[0056] (a) Making the fermentation base

[0057]Feathers are sterilized, and a culture solution is added in a certain proportion to obtain a fermentation base; the culture solution contains a carbon source, and each liter of the culture solution contains 15 g of a carbon source, and the carbon source sucrose; the culture solution contains Nitrogen source, containing nitrogen source 15g in every liter of described culture solution, described nitrogen source is corn steep liquor; Also contains inorganic salt in described culture solution, contains inorganic salt 0.3g in every liter of described culture solution, and described inorganic salt for manganese sulfate.

[0058] (b) Inoculate with bacteria that can degrade feathers

[0059] Inoculate the obtained fermented bottom material with a certain proportion of bacterial strains capable of degrading feath...

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Abstract

The invention discloses a bacillus paramycoides fermentation method. The fermentation method is characterized by comprising the following steps: (a) production of a fermentation substrate: sterilizingfeather, and adding a nutrient solution in a certain proportion to obtain the fermentation substrate; (b) inoculation with strains capable of degrading the feather: inoculating the obtained fermentation substrate with a certain proportion of the strains capable of degrading the feather; and (c) fermentation: carrying out fermentation at certain temperature and PH value for 20 H-80 H. The bacillusparamycoides fermentation method which can rapidly and efficiently degrade the feather is provided and can rapidly degrade feather, nutrient ingredients such as amino acid can be obtained after fermentation, the problem that the feather is difficult to treat is solved, and the fermentation process basically causes no environmental pollution.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a method for fermenting Bacillus pseudomycoides. Background technique [0002] In recent years, my country's poultry breeding industry has developed rapidly. Feathers can account for 5%-7% of the weight of broilers, and the annual output exceeds 1 million tons. Waste, and seriously pollute the environment, and even spread diseases, causing Newcastle disease, air bag inflammation and keratoconjunctivitis. health problems. At present, physical and chemical methods such as high temperature and high pressure, strong acid and alkali, and extrusion are usually used to treat feathers. The process is complex, consumes a lot of energy, and is easy to cause environmental pollution. Therefore, if feathers can be recycled, environmental pollution will be reduced, resources will be saved, and the sustainable development of my country's resources will be promoted. Contents of the inve...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/38C12P1/04C12R1/07
CPCC12N1/20C12N1/38C12P1/04C12R2001/07C12N1/205
Inventor 姜明国申乃坤杨立芳朱英芝王金子丰景王一兵
Owner GUANGXI UNIV FOR NATITIES
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