Microfluidic sample injection system

Abstract

The invention discloses a microfluidic sample injection system, comprising a quantitative sampling module, a pneumatic and control module and a negative-pressure generator, wherein the quantitative sampling module comprises a sample pool, an inlet working channel, a first one-way valve, a first drain valve, a quantitative sampling chamber, a second drain valve, a third drain valve, a second one-way valve and an outlet working channel; the first one-way valve is arranged on the inlet working channel; the second one-way valve is arranged on the outlet working channel; the first drain valve and the second drain valve are respectively arranged at the inlet end and the outlet end of the quantitative sampling chamber; the third drain valve is arranged between the second drain valve and the second one-way valve; the pneumatic and control module comprises a miniature air pump, a miniature electromagnetic valve and a controller; the air pump is an air source for providing driving pressure; andthe electromagnetic valve and the controller form a control unit to control on-off of driving pressure. According to the system, the sample injection precision of quantitative samples can be improved,the flow rate and the flow of the samples can be controlled according to a pulse modulation principle during sample injection, and sample injection cost can be saved.

Description

Technical field [0001] The invention belongs to the technical field of microfluidics, in particular to a microfluidic sampling system. Background technique [0002] Microfluidics is an intersecting science and technology involving fluid mechanics, precision machinery, biomedicine, chemistry and other fields. It has a wide range of applications in biochemical analysis, medical diagnosis, and drug screening. It has high efficiency and needs. The advantages of fewer samples / reagents and easy miniaturization of equipment design have led to rapid development in recent years. Fluid sampling is a key link in the engineering application of microfluidic technology, involving quantitative sampling, fluid drive and injection flow rate control. This operation can be accomplished by using a built-in integrated micropump, but the structure is complex and the cost is high, and it is not suitable for disposable microfluidic chips. If an external injection pump, pressure pump, etc. are used for...

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Problems solved by technology

For example Jessica Melin&Stephen R.Quake, Microfluidic Large-ScaleIntegration: The Evolution of Design Rules for Biological Automation (J), Annu.Rev.Biomol.Struct.2007.36:213-231. The method of controlling the opening and closing of the control channel to complete the quantitative sampling of micro samples. In order to realize online quantitative sampling, this method usually requires multiple control microvalves, the structure is relatively complicated, and the flow dead zone in the microvalve is likely to cause liquid residue, which affects the sampling accuracy.

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